Hyaluronan binding protein

Antibodies used for cell or embryo immunofluorescence were used at a dilution of 1/100 unless otherwise specified. Antibodies were raised against Isl1 (Developmental Hybridoma bank, Iowa University 1/50), Tbx20 (novus biological H00057057-B01), Msx1 (Abcam ab93287), Tbx3 (Santa Cruz sc-178721), aggrecan (Chemicon AB1031), versican (Chemicon AB1033), smooth muscle actin (Sigma A-2547), Filamin A (Epitomics, CA, USA), vimentin dylight 550 (Novus biological, VM452, 1/200), periostin (Abcam ab140141), CD31 (BD pharmigen, WM59), VE-cadherin (R&D systems/MAB9381), Sox9 (a gift from Pr Wegner, University of Nurnberg, and Santa-Cruz Sc17431), GATA5 (Abcam ab11877), Hyaluronan-binding protein (Millipore 385911), collagen I (Abcam 34710), anti-Patched1 (Merck-Millipore, France, 06–1102), human Lamin A/C (Novacastra, NCL-LAM-A/C), and anti-NFATc (Santa-Cruz 17844 H10).

C57BL/6 female mice at 5 days (d), 10 weeks (w), and 24 w of age were obtained from Institute of Laboratory Animal Sciences (CAMS&PUMC, Beijing, China). All experimental protocols were approved by the Institute of Materia Medica Animal Authorities (No. 00003787, 27 May 2019).
Antibodies to the following antigens were used: aggrecan (Millipore, AB1031, Burlington, MA, USA), CD44 (BioLegend, 103028, San Diego, CA, USA), Chondroitin Sulfate (Abcam, ab11570, Cambridge, UK), collagen I (Abcam, ab34710, Cambridge, UK), collagen II (Santa Cruz, sc-52658, Dallas, TX, USA), collagen IX (Santa Cruz, sc-376969, Dallas, TX, USA), collagen XI (Lifespan, LS-C-352032, Seattle, WA, USA), hyaluronan binding protein (Millipore, 385911, Burlington, MA, USA), osteocalcin (Santa Cruz, sc-376835, Dallas, TX, USA), syndecan-1 (BioLegend, 142511, San Diego, CA, USA), and donkey anti-rabbit (Jackson, 34213ES60, West Grove, PA, USA) and goat anti-mouse IgG2b (Thermo, A-21144, Waltham, MA, USA).