Calcein AM (CAM) (C34851), propidium iodide (PI) (P1304MP), hypoxia reagent (H10498), 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (NBDG), cell tracker red CMTPX (C34552), green CMFDA (C7025), and blue (C2110), were purchased from Thermo Fisher and stock solutions were prepared following supplier instructions. Rhodamine B (Rho) (Sigma R6626), Doxorubicin (DOX) (Selleckchem, S1208) and Tirapazamine (TPZ) (Sigma, SML0552) were dissolved at 1 mg/ml, 100 mM, and 50 mM respectively in DMSO.
Rhodamine b rho
Manufactured by Merck Group
Sourced in Germany, China, United States
Rhodamine B (Rho) is a fluorescent dye commonly used in various laboratory applications. It is a synthetic organic compound that exhibits strong absorption and emission properties in the visible light spectrum. Rhodamine B is often utilized as a tracer, marker, or stain in diverse scientific and research fields, including but not limited to cell biology, microscopy, and diagnostic testing.
Automatically generated - may contain errors
Lab products found in correlation
Doxorubicin dox, by Selleck Chemicals (1 mentions)
Tirapazamine tpz, by Merck Group (1 mentions)
Polyethylene glycol peg 400, by Merck Group (1 mentions)
Peg4000, by Merck Group (1 mentions)
Kolliphor el, by BASF (1 mentions)
Kolliphor p188, by BASF (1 mentions)
Phosphate buffer solution 1.0 m, by Merck Group (1 mentions)
Fluorescein flu, by Merck Group (1 mentions)
Ethanol, by Samchun (1 mentions)
Calcium chloride dihydrate, by Samchun (1 mentions)
β actin bsm 33036m, by Bioss Antibodies (1 mentions)
Amicon ultra centrifuge filter, by Merck Group (1 mentions)
Cytopainter lysored indicator reagent ab176828, by Abcam (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Abcam (1 mentions)
Fetal bovine serum (fbs), by Wisent (1 mentions)
Trypsin, by Wisent (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyl 2h tetrazolium bromide mtt, by Wisent (1 mentions)
Antibiotic antimycotic 100, by Wisent (1 mentions)
Analytical kit, by Nanjing Jiancheng (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
5 protocols using rhodamine b rho
1
Multimodal Cell Staining Protocol
2
Formulation of Lipid-Based Nanocarriers
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Dynasan 118 (tristearin) was obtained from IOI OLEO GmbH (Hamburg, Germany). Compritol 888 ATO and Gelucire®48/16 were kindly supplied from Gattefossè (Milan, Italy). Kolliphor® P188 (Poloxamer 188) and Kolliphor® EL, formerly known as Cremophor® EL (Polyoxyl 35 Hydrogenated Castor Oil), were a gift of BASF. Cetylstearyl alcohol, polyethylene glycol (PEG) 400, PEG 4000 and rhodamine B (Rho) were purchased from Sigma Aldrich (Steinheim, Germany). All other chemicals were of analytical grade. The properties of the hydrophobic and hydrophilic compounds are reported in Table 1 .
3
Silk Fiber Regeneration and Labeling
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Degummed B. mori silkworm silk fiber was supplied by BJ Silk Co. Ltd (Jinju-si, Korea). Calcium chloride dihydrate (71.0%–77.5%) and ethanol (94.5%) were purchased from Samchun Chemical (Pyeongtaek, Korea). Dialysis cellulose membranes were purchased from Sigma-Aldrich Co. (cutoff 12,000–13,000 Da) and Membrane Filtration Products Inc. (Cellu-Sep 5030-43, cutoff 3,500 Da) and presoaked in distilled water for 10 minutes before use. Polyethylene glycol was purchased from KPX Green Chemical Co. Ltd (Seoul, Korea). Phosphate buffer solution (1.0 M, pH 7.4), protease from Streptomyces griseus, rhodamine B (Rho), and fluorescein (Flu) were purchased from Sigma-Aldrich Co. All the chemical agents were used without further purification.
4
Nanoparticle-Mediated Anticancer Therapy
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FUdR was purchased from Alfa Aesar (Shanghai, China). FUdR15 strands were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). PHBHHx was purchased from Bluepha (Beijing, China). Rhodamine B (Rho) and Cur were purchased from Sigma-Aldrich (Shanghai, China). DAPI and CytoPainter LysoRed Indicator Reagent (ab176828) were obtained from Abcam Co., Ltd. (Cambridge, MA, USA). Trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), antibiotic-antimycotic (100×), and fetal bovine serum (FBS) were purchased from Wisent Biotechnology Co., Ltd. (Nanjing, China). All chemicals were used without further purification. Primary antibodies to Bcl-2 (bsm-33047M), Bax (bsm-33283M), and β-actin (bsm-33036M) were purchased from Bioss Antibodies. Caspase 3, 8, and 9 activity detection kits were purchased from BestBio Biotechnology Co., Ltd. (Shanghai, China). Amicon ultracentrifuge filters were purchased from Merck Millipore Ltd. (Darmstadt, Germany).
5
PLGA-mPEG Nanoparticles for Rapamycin Delivery
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Monomethoxy-poly(ethylene glycol) (mPEG, 5,000 Da) and poly(lactic-co-glycolic acid) (PLGA, molar ratio of D, L-lactic to glycolic acid, 75:25) were purchased from Jinan Daigang Biotechnology Co. Ltd. (Jinan, China). Rapamycin (RAPA, purity ≥99%), N,N-Diethylnicotinamide (DENA), polyvinyl alcohol (PVA), and Rhodamine B (RHO) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS), penicillin, and streptomycin were obtained from by Gibco (Grand Island, NY, USA). The bicinchoninic acid (BCA) protein assay kit was obtained from Beyotime Institute of Biotechnology (Beijing, China). Liver triglyceride and cholesterol contents were determined by analytical kit from Jiancheng Bioengineering (Nanjing, China). The other chemicals and reagents with used in this study were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Beijing, China) and were of analytical grade.
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