Antibodies used in this study are as follows: Sema7A (goat, 1:3000, #AF-1835, R and D Systems); PlxnC1 (goat, 1:3000, discontinued, Abcam); CNG-A2 (rabbit, 1:200, #APC-045, Alomone Labs); vGlut2 (guinea pig, 1:1000, #AB2251-l, Millipore); GluR1 (rabbit, 1:1000, #ab51092, Abcam); GFP (rabbit, 1:1000, #A-10260, Thermo Fisher Scientific); Lucifer yellow (rabbit, 1:2000, #A-5750, Thermo Fisher Scientific); and EGR1 (rabbit, 1:1000, #ab6054, Abcam).
Af 1835
Manufactured by R&D Systems
Sourced in France
The AF-1835 is a piece of laboratory equipment manufactured by R&D Systems. It is designed to perform a specific function, but without further details, I cannot provide a more detailed description while maintaining an unbiased and factual approach. Description not available.
Automatically generated - may contain errors
2 protocols using af 1835
1
Immunohistochemical Staining of Neuronal Markers
2
Immunohistochemical Staining of Reproductive Markers
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The rabbit polyclonal anti-GnRH (1:3,000) was a generous gift from Professor G. Tramu (Centre Nationale de la Recherche Scientifique, URA 339, Université Bordeaux I, Talence, France).
Antibodies were against Sema7A (goat polyclonal; 1:400; AF1835; R&D Systems), PlexinC1 (sheep polyclonal; 1:400; AF5375; R&D Systems), β1-Integrin (rabbit polyclonal; 1:500, sc-8978; Santa Cruz), vimentin (chicken polyclonal, 1:2,000, AB5733, Millipore), progesterone receptors A/B (rabbit polyclonal, 3176S; 1:1,000, Sigma) and BSL-1 (endothelial cell marker, TRITC-labelled Bandeiraea Simplicifolia Lectin BS-1 L5264, Sigma). A donkey anti-chick AlexaFluor 568-conjugated secondary antibody (1:500) was used for vimentin, the donkey anti-rabbit AlexaFluor 488-conjugated secondary antibody (1:500) for Sema7A and the donkey anti-mouse AlexaFluor 350-conjugated secondary antibody (1:500) for GnRH detection. Phalloidin (1:100) used to stain actin cytoskeleton of fixed and permeabilized cells was labelled with rhodamine and purchased from Invitrogen (Eugene, OR).
Antibodies were against Sema7A (goat polyclonal; 1:400; AF1835; R&D Systems), PlexinC1 (sheep polyclonal; 1:400; AF5375; R&D Systems), β1-Integrin (rabbit polyclonal; 1:500, sc-8978; Santa Cruz), vimentin (chicken polyclonal, 1:2,000, AB5733, Millipore), progesterone receptors A/B (rabbit polyclonal, 3176S; 1:1,000, Sigma) and BSL-1 (endothelial cell marker, TRITC-labelled Bandeiraea Simplicifolia Lectin BS-1 L5264, Sigma). A donkey anti-chick AlexaFluor 568-conjugated secondary antibody (1:500) was used for vimentin, the donkey anti-rabbit AlexaFluor 488-conjugated secondary antibody (1:500) for Sema7A and the donkey anti-mouse AlexaFluor 350-conjugated secondary antibody (1:500) for GnRH detection. Phalloidin (1:100) used to stain actin cytoskeleton of fixed and permeabilized cells was labelled with rhodamine and purchased from Invitrogen (Eugene, OR).
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