Facsaria ιιι flow cytometer

Manufactured by BD

The BD FACSAria III is a flow cytometer that allows users to analyze and sort individual cells or particles within a sample. It is designed to provide high-speed cell sorting and analysis capabilities for a variety of applications, including immunophenotyping, stem cell research, and cell biology studies.

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FACSAria (4019 citations) FACSAria flow cytometer (761 citations) FACSAria cytometer (130 citations)

2 protocols using facsaria ιιι flow cytometer

Spike-specific Memory B Cell Isolation

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B cells were purified magnetically (STEMCELL Technologies, 17954) and stained with anti-CD27-APC (Biolegend, 356410), anti-human IgM (Biolegend, 314512), anti-human IgG (Biolegend, 410708) and biotinylated spike protein. Spike-specific memory B cells were isolated by flow-cytometric sorting using a BD FACSAria ΙΙΙ flow cytometer (BD Biosciences) from pooled PBMCs. Flow-cytometric data were analyzed with FlowJo.

Dou Y., Xu K., Deng Y.Q., Jia Z., Lan J., Xu X., Zhang G., Cao T., Liu P., Wang X., Wang X., Xu L., Du P., Qin C.F., Liu H., Li Y., Wu G., Wang K, & Lu B. (2023). Development of neutralizing antibodies against SARS-CoV-2, using a high-throughput single-B-cell cloning method. Antibody Therapeutics, 6(2), 76-86.

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Isolation and Characterization of RVFV-Specific Memory B Cells

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Angola to China was diagnosed with RVF, representing the first imported case of RVFV infection in China 6 . On day 46 of the disease, blood was taken after written consent using guidelines approved by the ethics committee of the Beijing Ditan Hospital. Peripheral blood mononuclear cells were isolated following the manufacturer's instructions (GE Healthcare) before being subjected to cell sorting as previously reported 22, 36 . Briefly, peripheral blood mononuclear cells were incubated with both His-tagged Gn and His-tagged Gc at 100 nM before staining with anti-CD3, anti-CD16, anti-CD235a, anti-CD19, anti-CD38, anti-CD27 and anti-His. Antigen-specific memory B cells, which were CD3 -, CD16 -, CD235a -, CD38 -, CD19 + , CD27 + , hIgG + and His + were sorted into 96-well PCR plates with one cell per well. Flow cytometric analysis and cell sorting were performed on a BD FACSAria ΙΙΙ flow cytometer (BD Biosciences) and the data were analysed using FlowJo.

Wang Q., Ma T., Wu Y., Chen Z., Zeng H., Tong Z., Gao F., Qi J., Zhao Z., Chai Y., Yang H., Wong G., Bi Y., Wu L., Shi R., Yang M., Song J., Jiang H., An Z., Wang J., Yilma T.D., Shi Y., Liu W.J., Liang M., Qin C., Gao G.F, & Yan J. (2019). Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus. Nature microbiology, 4(7).

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