Acute exercise responses for blood glucose (Accu-Chek Aviva Plus Glucometer; Roche Diagnostic, Indianapolis, IN), β-hydroxybutyrate (Nova Blood Ketone Monitor; Nova Biomedical, Waltham, MA) and lactate (Lactate Plus Meter; Nova Biomedical, Waltham, MA) were measured via tail vein in conscious mice before and after acute exercise at the end of week 5 (Figure 1A ). Serum lipids were analyzed by measuring non-esterified fatty acids (Wako Chemicals, Richmond, VA) and triglycerides (Sigma, St. Louis, MO). ELISA kits were used to detect serum insulin (Mercodia, Uppsala, Sweden; Cat#10–1247-01) and serum corticosterone (Enzo Life Sciences, Farmingdale, NY; Cat# ADI-900–097). Manufacturer’s recommended protocols were used for all measurements.
Accu chek aviva plus glucometer
Manufactured by Roche
The Accu-Chek Aviva Plus Glucometer is a portable device used to measure blood glucose levels. It provides accurate and reliable results to aid in the management of diabetes.
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5 protocols using accu chek aviva plus glucometer
1
Acute Exercise Metabolic Responses in Mice
2
Glucose tolerance test in mice
3
Acute exercise metabolic responses
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Acute exercise responses for blood glucose (Accu-Chek Aviva Plus Glucometer; Roche Diagnostic, Indianapolis, IN), β-hydroxybutyrate (BHB) (Nova Blood Ketone Monitor; Nova Biomedical, Waltham, MA), and lactate (Lactate Plus Meter; Nova Biomedical, Waltham, MA) were measured via tail vein in conscious mice before and after acute exercise at the end of week 5 (Fig. 1 A). Serum lipids were analyzed by measuring nonesterified fatty acids (NEFA) (Wako Chemicals, Richmond, VA) and TAG (Sigma, St. Louis, MO). ELISA kits were used to detect serum insulin (Mercodia, Uppsala, Sweden; Cat 10-1247-01) and serum corticosterone (Enzo Life Sciences, Farmingdale, NY; Cat ADI-900-097). Manufacturer’s recommended protocols were used for all measurements.
4
Glucose Metabolism in WT and TB Mice
5
Glucose Tolerance Test in Mice
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A glucose tolerance test was conducted at 3 months of age. Mice were fasted for 4 hours with full access to water (preliminary studies showed that typical overnight fasting resulted in a significant loss of body fat in mice and thus was not used here). Glucose (20 mg/kg BW) was administered by i.p. injection in 0.9% saline, with the volume (~160 μL) adjusted for body weight to maintain a constant dose. Tail blood was collected by tail vein nicks before glucose challenge and again at 30, 60 and 120 min after injection. Blood glucose was measured using an Accu-Chek Aviva plus glucometer (Roche, Indianapolis, IN). The area under the curve (AUC) for the blood glucose levels over the 120 min monitored after injection was calculated using the linear trapezoidal rule. The percent change in blood glucose from baseline was also calculated.
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