Cysteamine was purchased from Tokyo Chemical Industry CO, Ltd. All of the other commonly used chemicals and solvents were purchased from commercial sources (Sigma-Aldrich, VWR, EMD, Novagen, Invtrogen, Fluka) and used without any further purification. Antibiotic (ampicillin Na salt, EMD) was prepared as a stock solution (100 mg mL−1 or 500 mg mL−1) and stored at −20 °C. Stock solution of ethidium homodimer (3 mM) was prepared and stored at −20 °C. Growth media (Luria−Bertani Broth, Miller) was prepared, sterilized, and stored based on the manufacture instruction. Agilent Technologies 1200 Series HPLC with diode array detector was used for reverse phase high performance liquid chromatography (RP-HPLC). The buffer system is as following: buffer A (water with 0.1% TFA), buffer B (90% acetonitrile, 10% water, 0.1% TFA). Mass spectra were obtained on either an API 3000 LC/MS-MS System (Applied Biosystems/MDS SCIEX) or an API 150EX system (Applied Biosystems/MDS SCIEX).
Api 150ex system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The API 150EX system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and semi-preparative applications. It features a modular design and supports a variety of detector options, enabling flexible and customizable configurations to meet diverse analytical needs.
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Lab products found in correlation
2 protocols using api 150ex system
1
Cysteamine Synthesis and Purification
2
Mass Spectrometry Analyses of Peptides and Proteins
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Matrix assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed on a Voyager-DE system (PerSeptive Biosystems, USA) and data were analyzed using Data Explorer software. Peptide samples were co-crystallized with α-cyano-4-hydroxycinnamic acid in 1∶1 acetonitrile (MeCN) to H2O with 0.1% trifluoroacetic acid (TFA). Electrospray ionization mass spectrometry (ESI-MS) of peptides was performed using an Agilent 1100 series LC pump outfitted with either an Agilent 6224 Time-of-Flight (TOF) LC/MS system or an API 150EX system (Applied Biosystems, USA) equipped with a Turbospray ion source. LC-ESI-MS of proteins and tandem mass spectrometry (MS/MS) of peptides were obtained from the UC Berkeley QB3/Chemistry Mass Spectrometry Facility. Protein bioconjugates were analyzed using an Agilent 1200 series liquid chromatograph (Agilent Technologies, USA) that was connected in-line with an LTQ Orbitrap XL hybrid mass spectrometer equipped with an Ion Max electrospray ionization source (ESI; Thermo Fisher Scientific, Waltham, MA). MS/MS analysis was accomplished with a using a Waters nanoAcquity ultra performance liquid chromatograph (UPLC)/quadrupole time-of-flight (Q-TOF) Premier instrument.
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