Prime 8

The results were statistically analyzed using SAS 8.0 software and expressed as mean ± standard deviation (n = 3). The significance of the main effects was determined using the one-way ANOVA procedure, and the determination of significant differences (p < 0.05) among the means was performed by Duncan’s multiple-range and Tukey test. The graphs were generated using GraphPad Prime 8.

SPSS 26.0 version software and GraphPad Prime 8 were used to statistical analysis. Date was presented as the mean ± SD. Differences between groups were analyzed using two-tailed Students’ t-test or unpaired t-test. χ² test was employed to analyzed immunohistochemical scored. p<0.05 was considered statistically significant.

Nine eight-week-old male SD (Sprague-Dawley) rats (240~260 g) were randomly allocated into three groups of five rats in each group. The three groups were the specific F(ab’)₂, Fab-PEG-Fab, and Fab-PEG, respectively. The rats were subjected to a single intraperitoneal injection with the samples at 1 mg/kg body weight. The blood samples were collected from the rats at 30 min, 1, 2, 6, 12, 24, 36, 48, 72, and 96 h after injection. The concentrations of F(ab’)₂ in the sera were determined by ELISA using an HRP-conjugated anti-F(ab’)₂ polyclonal antibody and a known concentration of F(ab’)₂ was taken as the standard. The T_1/2 and AUC were measured by GraphPad Prime 8.

Values were expressed as mean ± SD and were analysed by one-way ANOVA followed by the Bonferroni post hoc test by Graph Pad Prime 8.0 (La Jolla, USA). P value <0.05 was considered statistically significant.

All experiments were done in triplicates, and each test was repeated three times. Results were statistically analyzed using SPSS22.0 software and GraphPad Prime 8.0, and exhibited as mean ± SD. Student’s t-test and ANOVA was performed to compare the differences in two or more groups. Kaplan-Meier method was utilized to plot the overall survival curve. The relationship between miR-1271-5p and TIAM1 in OC was identified using Pearson’s correlation analysis. Gene set enrichment analysis (GSEA) enrichment was performed with GSEA (version2.2.2, http://www.broadinstitute.org/gsea). P < 0.05 was regarded as significance.

Statistical comparisons were performed using SPSS version 22 software. Graphpad prime 8.0 software was used for drawing the map. The results were presented as the means ± standard deviations (SDs). Further analyses were performed using unpaired, two-tailed Student’s t test and one-way or two-way analysis of variance (ANOVA) followed by Tukey’s post hoc test of honestly significant differences (two-tailed). Probability (p) values < 0.05 were considered statistically significant.

All variables are expressed as the mean ± SEM. Comparisons were performed by Tukey’s test or one-way ANOVA. A p-value less than 0.05 was considered statistically significant. Statistical analyses were performed using the SPSS 26.0 statistical package and GraphPad Prime 8.0.