Images of biofilms were acquired using a Leica SP5 confocal microscope (Leica Microsystems, Mannheim, Germany) with spectral detection and a Leica HCX PL APO CS 63x water objective (NA 1.2). Optical sectioning was performed in 0.13 μm steps. GFP was excited at 488 nm, fluorescence emission was detected between 500 and 560 nm, and images were recorded without differential interference contrast (DIC) optics. For visualization and processing of image data the Leica LAS AF Lite and ImageJ 1.46 software was used. Quantification and morphological analysis of image stacks was performed using the computer program COMSTAT1 (Heydorn et al., 2000 (link); Vorregaard, 2008 ).
Hcx pl apo cs 63x water objective
Manufactured by Leica
Sourced in Germany
The HCX PL APO CS 63x water objective is a high-performance objective lens designed for Leica microscopes. It provides a 63x magnification with a high numerical aperture, delivering excellent image quality and resolution. The objective is optimized for use with water immersion, making it suitable for a variety of applications that require aqueous environments.
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Lab products found in correlation
2 protocols using hcx pl apo cs 63x water objective
1
Confocal Microscopy Analysis of Biofilms
2
Confocal Imaging and Analysis of Biofilms
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Images of biofilms were acquired using a Leica SP5 confocal microscope (Leica Microsystems, Mannheim, Germany) with spectral detection and a Leica HCX PL APO CS 63x water objective (NA 1.2). Optical sectioning was performed in 0.13 μm steps. GFP as well as SYTO 9 was excited at 488 nm, fluorescence emission was detected between 500 and 560 nm, and images were recorded without differential interference contrast (DIC) optics. For visualization and processing of image data the Leica LAF and ImageJ 1.46 software was used. Quantification and morphological analysis of image stacks was performed using the computer program COMSTAT (http://www.comstat.dk ) (Heydorn et al., 2000; Vorregaard et al. ).
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