Agilent eclipse xde c18

Determination of EDCs was obtained through a HPLC system coupled to an UV–Vis detector (Agilent Technologies) and a reverse-phase column Agilent Eclipse XDE-C18 150 × 4.6 mm, 5 μ. A reverse-phase column Agilent Eclipse XDE-C18 150 × 4.6 mm, 5 μ, was used for the chromatographic measurements. The final reaction mixture was performed at 25 °C in 1 mL vial containing 10 mg L⁻¹ of each analyte, 10 % (v/v) buffer McIlvain pH 5 and 100 U L⁻¹ (50.3 U mg⁻¹) of laccase; then the solution was vortex-mixed briefly for homogenizing and sheltering from light. The enzymatic treatments were measured by triplicate. An injection volume of 20 μL and 1 mL min⁻¹ gradient elution by means of (A) acetonitrile (ACN) and (B) 10 mM phosphate buffer (pH 3.5) were applied. The gradient program was set as follows: 0–11 min, 25 % (A); 11–23 min, 95 % (A) and 23–30 min, 25 % (A). BPA, NP, TCS and EE2 were detected at three wavelengths, 206, 290 and 275 nm. The chromatographic analysis was carried out up to 12 h and the extent of the reaction was estimated by the decrease of the corresponding analyte peak analyzed by HPLC-UV chromatography technique and quantified using a calibration curve.