Cation analysis kit

Manufactured by AB Sciex

Sourced in Germany, United States

The Cation Analysis Kit by AB Sciex is a laboratory equipment designed for the analysis of cations. It provides the essential components and tools required for the identification and quantification of various cationic species in samples.

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Lab products found in correlation

P ace mdq, by Beckman Coulter (1 mentions)

2 protocols using cation analysis kit

Capillary Electrophoresis for Ammonium Analysis

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For determination of ammonium, capillary electrophoresis (P/ACE MDQ, Beckman & Coulter) was employed, which is known to constitute a highly efficient and effective separation method for analysis of ionic species in general and thus also ammonium in particular [12 (link)–15 (link)]. The capillary was made of fused silica and had an effective length of 50 cm with an inner diameter of 75 µm and outer diameter of 375 µm. The samples were diluted 50-fold with ultrapure water. If no ammonium could be detected using this dilution factor, measurement was repeated with a 10-fold dilution. The volume was injected into the capillary using pressure injection, and separation took place at 30 kV with normal capillary polarity. Measurements were executed using a Cation Analysis Kit (ABSciex, Darmstadt, Germany). Indirect detection was performed with the help of a diode array analyzer at 200 nm. The temperature of tray and capillary was set to 25°C. The measurements were performed by one person only who underwent a detailed initial training program previously.
The absolute amounts of ammonium were calculated by taking the total urine volume of which the sample was taken into consideration.

Roehrborn F., Dohle D.S., Waack I.N., Tsagakis K., Jakob H, & Teloh J.K. (2017). Postoperative Compensatory Ammonium Excretion Subsequent to Systemic Acidosis in Cardiac Patients. BioMed Research International, 2017, 5383574.

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Capillary Electrophoresis for Urinary Ammonium and Phosphate

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For ammonium quantification, capillary electrophoresis was used. For this purpose, a fused silica capillary was employed with an effective length of 50 cm, an I.D. of 75 µm and an O.D. of 375 µm. Samples of initial urine were diluted with ultrapure water 1:50, whereas samples of the phase of dilution as well as observation phase were diluted 1:100. Analysis was performed using a cation analysis kit (ABSciex, Fullerton, USA). Per analysis, the volume was applied using pressure injection. The subsequent separation proceeded using a voltage of 30 kV and normal polarity of the capillary. Indirect detection was performed employing a photo diode array at a wavelength of 200 nm.
Quantification of phosphate in urine samples was realized with the help of a colorimetric test at a wavelength of 340 nm (Daly and Ertingshausen 1972) .
Here, inorganic phosphate reacts with ammonium molybdate to form an unreduced phosphomolybdate complex. The obtained results had already been corrected for background absorption by individual blank measurements that were automatically subtracted from the sample's absorbance by the analyzer.

Teloh J.K., Waack I.N, & de Groot H. (2017). Prompt rise in urinary ammonium excretion suffices to mitigate metabolic acidosis in an experimental animal model of severe normovolemic hemodilution. Physiological research, 66(4).

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