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5 protocols using peg 400

1

Topical Formulation Development and Characterization

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SN was purchased from 2A Pharmachem (Lisle, IL, USA). PEG 400 and propylene glycol were provided by BASF (Tarrytown, NY, USA). Klucel® (hydroxypropylcellulose) was provided by Ashland Inc. (Bridgewater, NJ, USA) while isopropyl myristate was from Croda Inc. (Edison, NJ, USA). Ascorbic acid and menthol were purchased from Sigma Aldrich (St. Louis, MO, USA) while glycerin was from Fisher Scientific (Waltham, MA, USA). Anhydrous ethanol (200 proof) was purchased from Acros Organic (Morris Plains, NJ, USA). All other reagents used were of high purity or HPLC grade.
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2

Hydrogel Formulation for Ophthalmic Delivery

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The following materials were used in this study: poloxamer Kolliphor P407 (BASF SE, Ludwigshafen, Germany); poloxamer Kolliphor P188 (BASF SE, Ludwigshafen, Germany); hyaluronic acids PrincipHYAL (1400–1800), PrincipHYAL (400–600), and PrincipHYAL (Cube3) (ROELMI.HPC, Origgio, Italy); PEG 1500 (BASF SE, Ludwigshafen, Germany); PEG 400 (BASF SE, Ludwigshafen, Germany); polyvinylpyrrolidone-10000 (PVP) (BASF SE, Ludwigshafen, Germany); ascorbic acid (Acros Organics, Geel, Belgium); cefuroxime (Kraspharma, Krasnoyarsk, Russia); food agar (Dr. Oetker, Belgorod, Russia); sodium polyacrylate (RusHim, Moscow, Russia); and food coloring (Kreda, Izhevsk, Russia).
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3

Polyacrylic Acid-Enhanced Ultrafiltration Membranes

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Ultrafiltration membranes were prepared via NIPS from casting solutions containing polysulfone (PSF, Ultrason S 6010, Mn = 55,000 g·mol−1, BASF) with the addition of polyethylene glycol (Mn = 400 g·mol−1, PEG-400, BASF) in N,N-dimethylacetamide (DMAc, 99%, BASF). Polyacrylic acid (PAA, Sigma-Aldrich, St. Louis, MO, USA) of three molecular weights (100,000, 250,000 and 450,000 g·mol−1) was applied as an additive to precipitation bath. PAA concentration was varied from 0 to 2.0 wt.%.
Polyvinylpyrrolidone (PVP K-30, Mn = 40,000 g·mol−1, Sigma-Aldrich, St. Louis, MO, USA) and lysozyme (Mn = 14,300 g·mol−1, pI = 11.35, Sigma-Aldrich, St. Louis, MO, USA) were applied as test substances to determine membrane separation properties. The solution of fertilizer (Hydrohumin, Biochem, Svisloch, Belarus) in tap water was used as a source of humic acids and applied for membrane separation and antifouling performance studies. The content of humic acids in the aqueous solution was 0.005 wt.%.
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4

Fabrication of Polysulfone-based Membranes

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Polyphenylsulfone (PPSU, Ultrason P 3010, BASF, Ludwigshafen, Germany, Mw = 4.8 × 104 g∙mol−1) and polysulfone (PSF, Ultrason S 6010, BASF, Mw = 4.5–5.5 × 104 g∙mol−1) were used for polymer solution and membrane preparation. The structures of the polymers are shown in Figure 1. Polysulfones were dried at 120 °C for 8 h in a drying oven prior to processing. PES Ultrason E 6020P PES (Mn = 1.1 × 104 g∙mol−1 , Mw = 5.8 × 104, BASF, Germany) was applied for the preparation of 1 wt.% solutions in N-methyl-2-pyrrolidone (NMP) to determine the coagulation values of different non-solvents.
Polyethylene glycol (PEG-400, BASF, Ludwigshafen, Germany) with Mn = 400 g∙mol−1 was used as the additive to the polymer solution, and NMP (EKOS-1, Russia) was utilized as the solvent without any further purification.
Human serum albumin (HSA, Mw = 6.7 × 104 g∙mol−1, isoelectric point of pH 4.7) was supplied by Sigma Aldrich. Anhydrous sodium monobasic phosphate (KH2PO4) and sodium dibasic phosphate trihydrate (K2HPO4∙3H2O) were purchased from Merck and used for the preparation of phosphate buffer solutions (0.05 M, pH = 7.0). MilliQ water was used throughout this study.
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5

Lipid-based Nanomedicine Preparation

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GRVRRSC (G: glycine; R: arginine; V: valine; S: serine; C: cysteine) was provided by GL Biochem Co., Ltd. (Shanghai, China). MonomethoxyPEG (mPEG) was provided by Sigma-Aldrich (St. Louis, MO). 4-Dimethylaminopyridine (DMAP), 1, 1-dioctadecyl-3, 3, 3, 3-tetramethylindodicarbo cyanine iodide (DiD) and Triethylamine (TEA) were all provided by Aladdin Co., Ltd. (Shanghai, China). Octadecanol, succinic anhydride, maleic anhydride, and tripterine (purity >98.0%) were all purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Coix seed oil (extracted by carbon dioxide supercritical technology, purity >85%). RH 40 and PEG 400 were purchased from BASF Co., Ltd. (Ludwigshafen, Germany). Dipalmitoylphosphatidylcholine (DPPC), stearoyllysophosphocholine (S-lysoPC), and Distearoylphosphoethanolamine-PEG2000 (DSPE-MPEG2k) were provided by A.V.T Co., Ltd. (purity > 98.0%, China). Double-distilled water was purified via Milli Q purification system (Merck Millipore). All other reagents used were of analytical grade.
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