Trans-well assays were performed to investigate the effects of OA-RD17 (1 nM) on the migration capacity of keratinocytes, macrophages, primary macrophages, and primary keratinocytes according to previous research [2 (link)]. The effects of miR-632 mimic (50 nM), miR-632 inhibitor (100 nM) (riboFECT™ CP Transfection Kit, RiboBio, China), and OA-RD17 (1 nM) on keratinocyte migration were determined following the manufacturer’s instructions. Additionally, the mechanism of OA-RD17-induced macrophage migration was investigated using a specific MAPK signaling pathway inhibitor (10 μM, HY-N1966, MedChemExpress, USA).
Hy n1966
Manufactured by MedChemExpress
Sourced in United States
HY-N1966 is a laboratory equipment product offered by MedChemExpress. It is a device used for measuring and analyzing various chemical and biological samples. The core function of HY-N1966 is to provide accurate and reliable data for researchers and scientists working in the field of life sciences and chemistry.
Automatically generated - may contain errors
4 protocols using hy n1966
1
Investigating Cell Migration Modulation
2
Keratinocyte Scratch Assay with OA-RD17, miR-632, and Inhibitors
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Cell scratch assays were performed to investigate the effects of OA-RD17 (1 nM), miR-632 mimic (50 nM), and miR-632 inhibitor (100 nM) (riboFECT™ CP Transfection Kit, RiboBio, China) on keratinocyte scratch repair following previous study [16 (link)]. In addition, the underlying mechanisms of OA-RD17- and miR-632-induced keratinocyte scratch healing were determined using a specific TLR4 inhibitor (1 μM, HY-11109, MedChemExpress, USA), MAPK signaling pathway inhibitor (1 μM, HY-N1966, MedChemExpress, USA), or β-catenin inhibitor (10 μM, HY-120697, MedChemExpress, USA).
3
Inhibition of FASN in Colorectal Cancer
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For the application of the FASN synthetase inhibitor Orlistat (O4139-25MG, Merck, Darmstadt, Germany) in vitro, the inhibitor was prepared by referencing the procedure of Browne et al. [17 (link)], and 200 µmol/L Orlistat was adopted to treat CRC cells for 48 h when necessary. Another FASN inhibitor, C75 (25–200 µmol/L) (HY-12364, MedChemExpress, Monmouth Junction, NJ, USA), was used to treat CRC cells for 48 h as per a report from Chen et al. [11 (link)]. Based on a previous study [18 (link)], 800 µmol/L of palmitoleic acid (HY-N1966, MedChemExpress) was applied for 72 h for in vitro experiments.
4
Proliferative Effects of OA-RD17 and miR-632 on Skin Cells
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The pro-proliferative effects of OA-RD17 (1 nM) on keratinocytes, macrophages, primary macrophages, and primary keratinocytes were determined according to previous study [16 (link)]. The effects of miR-632 mimic (50 nM), and miR-632 inhibitor (100 nM) (riboFECT™ CP Transfection Kit, RiboBio, China) on keratinocyte proliferation were explored according to the manufacturer’s instructions. The underlying mechanism of OA-RD17-induced proliferation against keratinocyte and macrophage was investigated using a specific MAPK signaling pathway inhibitor (10 μM, HY-N1966, MedChemExpress, USA). Furthermore, the mechanisms related to OA-RD17- and miR-632-induced proliferation of keratinocyte were detected using a specific TLR4 inhibitor (1 μM, HY-11109, MedChemExpress, USA) or β-catenin inhibitor (10 μM, HY-120697, MedChemExpress, USA).
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