Coilin

Manufactured by Proteintech

Sourced in United States

Coilin is a protein that serves as a structural component of Cajal bodies, which are nuclear organelles involved in the biogenesis and modification of small nuclear ribonucleoproteins (snRNPs). Coilin plays a crucial role in the organization and function of Cajal bodies.

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Lab products found in correlation

P3xflag cmv 10, by Merck Group (1 mentions) Gapdh, by Proteintech (1 mentions) Ta 08, by ZSGB-BIO (1 mentions) Gapdh, by ZSGB-BIO (1 mentions)

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Anti-coilin (2 citations)

2 protocols using coilin

Molecular Constructs for RNA Binding Proteins

Cited 2 times

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To construct the Flag-MTR4, Flag-RBM7 and Flag-ZCCHC7, the coding sequence of the corresponding gene was inserted into p3xFlag-CMV-10 (Sigma). Mutagenesis was used to obtain Flag-MTR4 mutant expression plasmids. Plasmids encoding β-globin cDNA (cG), Smad cDNA (cS) were described previously (20 (link),21 (link)). Speckle-targeting element (STE) sequence was inserted into the 3′ of β-globin cDNA to construct β-globin cDNA-STE (cG-STE).
Antibody to UAP56, CBP80 and ARS2 were described previously (9 (link),20 (link)). The rabbit polyclonal antibodies against MTR4 and MTR3 were purchased from ABclonal Technology. The Tubulin, RRP6, RRP40, Flag and SC35 antibodies were purchased from Sigma, the PAPD5, digoxin, GAPDH, ZCCHC8 and Coilin, PSP1 and PML antibodies were purchased from Protein tech, Roche, Abcam, Dundee cell, SANTA CRUZ, respectively.

Fan J., Kuai B., Wang K., Wang L., Wang Y., Wu X., Chi B., Li G, & Cheng H. (2018). mRNAs are sorted for export or degradation before passing through nuclear speckles. Nucleic Acids Research, 46(16), 8404-8416.

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Western Blot Analysis of Key Proteins

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Western blot analyses were performed as earlier described. Briefly, samples of cell lysates were prepared and separated by 10% SDS–polyacrylamide gel electrophoresis (SDS-PAGE), then transferred onto polyvinylidene fluoride (PVDF) filters. The probing antibodies were against the following antigens: UHMK1 (SC-393605, Santa Cruz Biotechnology, Santa Cruz, CA, USA), coilin (10967-1-AP, Proteintech Group, Rosemont, USA) and GAPDH (TA-08, ZSGB-BIO, Beijing, China).

Niu H., Zhao M., Huang J., Wang J., Si Y., Cheng S, & Ding W. (2022). UHMK1-dependent phosphorylation of Cajal body protein coilin alters 5-FU sensitivity in colon cancer cells. Cell Communication and Signaling : CCS, 20, 18.

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