Goat anti pdx1 antibody

Manufactured by R&D Systems

The Goat anti-PDX1 antibody is a laboratory reagent used for the detection and analysis of the transcription factor PDX1 (Pancreatic and Duodenal Homeobox 1) in biological samples. PDX1 is a key regulator of pancreatic development and function, and is commonly used as a marker for pancreatic cells. This antibody can be utilized in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to identify and study the expression of PDX1 in research settings.

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Lab products found in correlation

Goat anti sox17 antibody, by R&D Systems (2 mentions) Hoechst, by Merck Group (2 mentions) Goat anti oct4 antibody, by Santa Cruz Biotechnology (2 mentions) Rabbitanti foxa2 antibody, by Merck Group (2 mentions) Goatanti foxa2 antibody, by R&D Systems (2 mentions) Mouse anti aat antibody, by Thermo Fisher Scientific (2 mentions) Rabbit anti nanog antibody, by Abcam (2 mentions) Mouse anti afp antibody, by Merck Group (2 mentions) Mouse anti ck18 antibody, by Abcam (2 mentions) Goat anti albumin antibody, by Fortis Life Sciences (2 mentions) Alexa fluor 488 555 donkeyanti mouse, by Thermo Fisher Scientific (2 mentions) Anti rabbit or anti goat igg, by Thermo Fisher Scientific (2 mentions) Eclipse te2000 u microscope, by Nikon (2 mentions) Goat anti sox2, by Santa Cruz Biotechnology (1 mentions) Goat anti oct3 4, by Santa Cruz Biotechnology (1 mentions) Mouse anti tra 1 81, by Merck Group (1 mentions) Mouse anti ssea4, by Cell Signaling Technology (1 mentions) Mouse anti tra 1 60, by Cell Signaling Technology (1 mentions) Tcs sp5 laser scanning microscope, by Leica (1 mentions) Rabbit anti foxa2, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Goat anti-PDX1 Anti-PDX1

3 protocols using goat anti pdx1 antibody

Immunostaining Protocol for Lineage Markers

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Standard immunostaining was carried out as previously reported^{13 (link)}. Primary antibodies were goat
anti-SOX17 antibody (R&D, Cat # AF1924) diluted 1: 1,000, rabbit
anti-FOXA2 antibody (Millipore, Cat # AB4125) diluted 1:500, goat
anti-FOXA2 antibody (R&D, Cat # AF2400) diluted 1:1,000, mouse
anti-HNF4α antibody (Perseus Proteomics, Cat # PP-H1415-00)
diluted 1:500, mouse anti-AAT antibody (Thermo Scientific, Cat #
MA190438) diluted 1:500, goat anti-PDX1 antibody (R&D, Cat #
AF2419) diluted 1:1,000, goat anti-OCT4 antibody (Santa Cruz Biotechnology, Cat
# sc-8629) diluted 1:500, rabbit anti-NANOG antibody (Abcam, Cat
# ab80892) diluted 1:500, mouse anti-AFP antibody (Sigma-Aldrich, Cat
# A8452) diluted 1:500, mouse anti-CK18 antibody (Abcam, Cat #
ab82254) diluted 1:500, and goat anti-albumin antibody (Bethyl, Cat #
A80-129A) diluted 1:500. Secondary antibodies were Alexa Fluor 488/555 donkey
anti-mouse or anti-rabbit or anti-goat IgG (1:1,000) (Invitrogen). Nuclei were
visualized by Hoechst (Sigma-Aldrich) staining. Images were captured using a
Nikon Eclipse TE2000-U microscope.

Zhu S., Rezvani M., Harbell J., Mattis A.N., Wolfe A.R., Benet L.Z., Willenbring H, & Ding S. (2014). Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature, 508(7494), 93-97.

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Immunostaining Protocol for Lineage Markers

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Immunofluorescence Characterization of Stem Cells

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Cells were fixed with 4% paraformaldehyde for 30 min and then permeabilized in PBS containing 0.2% Triton X-100. Cells were blocked with PBS containing 3% BSA, and incubated with primary antibodies overnight at 4 °C. Then secondary antibodies were incubated for 1 h at room temperature after washing with PBS. Images were acquired on a TCS SP5 laser-scanning microscope (Leica). The following antibodies and dilutions were used: goat anti-OCT-3/4 (1:500, #sc-8628, Santa Cruz), goat anti-SOX2 (1:500, #sc-17320, Santa Cruz), mouse anti-TRA-1-60 (1:1000, #4746, Cell Signaling), mouse anti-TRA-1-81 (1:50, MAB4381, Millipore), mouse anti-SSEA4 (1:500, #4755, Cell Signaling), rabbit anti-FOXA2 (1:250, #8186, Cell Signaling), goat anti-SOX17 antibody (1:500, #GT15094, Acris/Novus), goat anti-PDX1 antibody (1:500, #AF2419, R&D Systems).

Wang X., Sterr M., Burtscher I., Chen S., Hieronimus A., Machicao F., Staiger H., Häring H.U., Lederer G., Meitinger T., Cernilogar F.M., Schotta G., Irmler M., Beckers J., Hrabě de Angelis M., Ray M., Wright C.V., Bakhti M, & Lickert H. (2018). Genome-wide analysis of PDX1 target genes in human pancreatic progenitors. Molecular Metabolism, 9, 57-68.

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