Bv421 conjugated anti cd64

Isolated cells were resuspended in 2% FBS/PBS solution and blocked with FcBlock on ice. Immune cell subpopulations were identified by staining for 20 minutes at room temperature with the following antibodies: Alexa Fluor 647–conjugated anti-CD45 (1:100; eBioscience; catalog 17-0112); FITC-conjugated anti-CD11b (1:100; eBioscience; catalog 17-0112); phycoerythrin-(PE)–conjugated anti-F4/80 (1:100; eBioscience; catalog 12-4801 or 53-4801); BV421–conjugated anti-Ly6G (1:100; BioLegend; catalog 141709); APC-conjugated anti-Ly6C (1:20; BioLegend; catalog 117326); BV421-conjugated anti-CD64 (1:100; BioLegend, catalog 139309); Alexa Fluor 700–conjugated anti-CD206 (1:100; BioLegend, catalog 141734); PE-Cy7–conjugated anti-MerTK (1:100; eBioscience, catalog 25-5751-82); BV421-conjugated anti-CD120a (1:100; R&D Systems, catalog FAB5538P); APC-Cy7–conjugated anti-Csf1r (1:100; BioLegend; catalog 135531, anti-Tmem173 (1:200; Abcam; catalog ab92605); and Alexa Fluor 488– (1:400; Invitrogen; catalog A21206) and APC-conjugated anti-CD120b (rat, 1:100; eBioscience; catalog 45-5932). 7AAD was added prior to cell analyses to exclude dead cells. Flow cytometric analyses and cell sorting were performed using a BD FACSAriaIII flow cytometer (BD Biosciences) and FlowJo software (Tree Star).