Sta ptt automated reagent

The anticoagulant activity was determined with prothrombin time (PT) and activated partial thromboplastin time (APTT) following the standard method described by [21 ]. 250 μL of human platelet-poor-plasma was incubated in a reaction vial with 100 μL of different concentrations of BVME, BVPE, BVAE, TPME, TPPE, TPME, OSME, OSPE, and OSAE (50, 25, and 12.5 mg/mL, respectively) for 7 minutes at room temperature before being subjected to PT and APTT tests.
Neoplastin Cl Plus reagent and STA-PTT automated reagent, Diagnostica Stago (Asnieres-sur-Seine, France), were reconstituted and subjected to PT and APTT assays, together with 0.025M calcium chloride. The PT and APTT assays were analysed using STA Compact coagulation analyser (Diagnostica Stago, France) according to the manufacturer's protocol. The time taken for clot formation was detected using the analyser and compared with the PT and APTT from control (without extracts) as the negative control and plasma mixed with heparin sodium salt (140 IU/mg) (Sigma-Aldrich, USA) as the positive control. All experiments were carried out in triplicate.

Standard human normal pooled plasma, STA-PTT automated reagent, and 0.025 M calcium chloride were purchased from Diagnostica Stago (Asnieres-sur-Seine, France), while sodium bicarbonate, sodium borohydride, gallic acid, carbazole, glucuronic acid, arabinose, galactose, mannose, rhamnose, xylose, dextran standards, trifluoroacetic acid (TFA), and heparin sodium salt were bought from Sigma-Aldrich (Missouri, USA). Phenol, acetonitrile, ammonium hydroxide, glacial acetic acid, acetic anhydride, dimethyl sulfoxide (DMSO), and Folin-Ciocalteu's reagent were purchased from Merck (New Jersey, USA). Concentrated sulphuric acid and glucose were purchased from Fisher Scientific (Massachusetts, UK), and sodium borate de carbohydrate was purchased from Biobasic (Ontario, Canada).