LO2, HepG2, MHCC97H, HCCLM3, and SMMC-7721 cell lines were purchased from the Institute of Chemistry and Cell biology (Shanghai, China). Hep3B, HCCC-9810, BEL-7404, QGY-7701, and Huh7 were purchased from the American Type Culture Collection (Manassas, VA). HCC cell lines including Hep3B, SMMC-7721, HCCC-9810, HepG2, BEL-7404, HCCLM3, QGY-7701, Huh7, and MHCC97H were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (HyClone, Logan, UT, USA). Normal liver epithelial cells—LO2—were maintained in a bronchial epithelial growth medium (Clonetics Corporation, Walkersville, MD), supplemented with 5 ng/mL epithelial growth factor, 70 ng/mL phosphorylethanolamine, and 10% fetal bovine serum. Cells were maintained in a humidified atmosphere at 37 °C with 5% CO2.
Qgy 7701
Manufactured by Thermo Fisher Scientific
Sourced in United States
The QGY-7701 is a compact and versatile laboratory equipment designed for various scientific applications. It features high-precision performance and reliable operation to meet the needs of research and development laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Thermo Fisher Scientific (5 mentions)
Smmc 7721, by Thermo Fisher Scientific (4 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Hep3b, by American Type Culture Collection (2 mentions)
Hcclm3, by Thermo Fisher Scientific (2 mentions)
Hep3b, by Thermo Fisher Scientific (2 mentions)
Sk hep1, by Thermo Fisher Scientific (2 mentions)
Mhcc97h, by Thermo Fisher Scientific (1 mentions)
Hccc 9810, by Thermo Fisher Scientific (1 mentions)
Bel 7404, by Thermo Fisher Scientific (1 mentions)
Bel 7404, by American Type Culture Collection (1 mentions)
Qgy 7701, by American Type Culture Collection (1 mentions)
Hccc9810, by American Type Culture Collection (1 mentions)
Bel 7402, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Merck Group (1 mentions)
Hepg2, by Shanghai Cell Bank (1 mentions)
Qgy7701, by Shanghai Cell Bank (1 mentions)
5 protocols using qgy 7701
1
Characterization of Liver Cancer Cell Lines
2
Cultivation of Liver Cancer Cell Lines
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The liver cancer cell lines, SMMC7721, QGY-7701, Bel-7402, PLC5, Huh7, HCCLM3, HepG2, and the normal liver cell line, LO2, were obtained from the Shanghai Cell Bank (Shanghai, China). SMMC7721, QGY-7701, Bel-7402 and PLC5 cells were cultured in RPMI-1640 (Invitrogen, Carlsbad, CA). Huh7, HCCLM3, HepG2 and LO2 cells were grown in DMEM (Invitrogen). All media were supplemented with 10% fetal calf serum (Invitrogen) and 100 IU/ml penicillin (Sigma, St. Louis, MO).
3
Characterization of HCC and Hepatocyte Cell Lines
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Human HCC cell lines QGY-7701, SMMC-7721, and HepG2 and the human hepatocyte cell line LO2 were purchased from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, P.R. China). The QGY-7701, SMMC-7721, HepG2, and LO2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS) and incubated at 37°C in a 5% CO2 humidified incubator.
4
Cultivation of Human and Mouse Liver Cancer Cell Lines
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Human HCC cell lines SK-Hep-1, HepG2, and Hep3B and human embryonic kidney cell line HEK293T were obtained from American Type Culture Collection (ATCC). SMMC-7721 was obtained from Second Military Medical University. Human HCC cell lines QGY-7701 and BEL-7401 and mouse liver cancer cell line Hepa1-6 were purchased from the Chinese Academy of Science (Shanghai, China). SK-Hep-1, SMMC-7721, Hepa1-6, QGY-7701, and BEL-7401 were cultured with DMEM (Gibco). Hep3B and HepG2 were cultured in MEM (Gibco). All mediums were supplemented with 10% fetal bovine serum (FBS), 100 IU/mL penicillin, and 100 mg/mL streptomycin (Gibco). Fludarabine was purchased from Selleck.
5
Comparative Analysis of Hepatocellular Carcinoma Cell Lines
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Human hepatocellular carcinoma cell lines (HepG2, QGY7701, QGY7703, QSG7701, Huh-7, MHcc97L, MHcc97H and SK-HEP-1), one mouse hepatocellular carcinoma Hepal-6 cell line and immortalized normal human liver HL7702 and L-02 cell lines were used in this study. HepG2, QGY7701, Hepal-6 and QGY7703 cell lines were obtained from Shanghai Maternal and Child Health Hospital (Shanghai, China). L-02, MHcc97L, MHcc97H, QSG7701, Huh-7 and SK-HEP-1 cell lines were purchased from the Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). The HL7702 cell line was obtained from the Chinese Academy of Science (Kunming, China). HepG2, QSG7701, L-02, HL7702 and Hepal-6 cells were maintained in RPMI-1640 medium (Gibco-BRL, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco-BRL). QGY7701, QGY7703, Huh-7, SK-HEP-1, MHcc97L and MHcc97H cells were maintained in Dulbecco’s modified Eagle’s medium (Gibco-BRL) supplemented with 10% FBS. All cell lines were cultured at 37°C in a humidified incubator with an atmosphere of 5% CO2.
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