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4 protocols using rabbit polyclonal anti ki67 antibody

1

Antibody Characterization for Research

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Mouse anti-VSVG polyclonal antibody and rabbit anti-NHE3 polyclonal antibody were previously described [22 (link)]. Rabbit anti-KLF5 polyclonal antibody was a kind gift of Dr. Jonathan Katz (Univ. of Pennsylvania). Rabbit polyclonal anti-Ki67 antibody was obtained from Leica (Newcastle, UK). Rabbit monoclonal anti-cleaved caspase-3 antibody was obtained from Cell Signaling Technology (Danvers, MA). Rabbit monoclonal anti-lysozyme antibody and rabbit polyclonal anti-chromogranin (CgA) antibody were obtained from Abcam (Cambridge, MA).
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2

Antibody Sources for Protein Analysis

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Antibodies were obtained from the following sources: mouse monoclonal anti‐GFP antibody from Wako; mouse anti‐β‐actin monoclonal antibody from Sigma; mouse anti‐Smad2/3 antibody from BD Transduction Laboratories (San Jose, CA, USA); rabbit polyclonal anti‐Smad4 and anti‐ALK5 antibodies from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit polyclonal anti‐cleaved caspase‐3 antibody from Cell Signaling Technology (Danvers, MA USA); and rabbit polyclonal anti‐Ki‐67 antibody from Leica (Newcastle, UK). Rabbit polyclonal anti‐phosphorylated Smad2 (PS2) and anti‐Smad2 antibodies were prepared in‐house.12
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3

Antibodies Used for Protein Expression Analyses

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The following antibodies were purchased and used as recommended by their suppliers: rabbit polyclonal antibodies against keratin 1, keratin 5, keratin 10, and keratin 14 (Covance, Emeryville, CA, USA); mouse anti-α-tubulin monoclonal antibody and mouse anti-β-actin monoclonal antibody (Sigma, St. Louis, MO, USA); rabbit anti-Ki67 polyclonal antibody (Novocastra, Wetzlar, Germany); rabbit anti-Runx1 monoclonal antibody, rabbit anti-Runx3 monoclonal antibody (MBL, Tokyo, Japan); and fluorescein isothiocyanate (FITC)-conjugated and horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG antibodies (Amersham, Tokyo, Japan). Secondary antibodies conjugated with Alexa Fluor dyes were purchased from Life Technologies (Thermo Fisher, Waltham, MA, USA).
Anti-panRunx antibody was described previously [28 (link)].
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4

Comprehensive Antibody Characterization for Cell Lineage Tracing

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Antibodies used in this study are in the followings: mouse anti-E-cad (BD Transduction Laboratories 61081, 1:250); chick anti-GFAP (Millipore, 1:5,000); chick anti-GFP IgY (Abcam ab13970, 1:5,000); rabbit anti-GLAST polyclonal antibody (Covalab (France) PA-INS, 1:1,000); rabbit anti-Ki67 polyclonal antibody (Novocastra, NCL-Ki67p, 1:1,000); goat anti-NeuroD antibody (N-19; Santa Cruz, sc-1,084, 1:1,000); rabbit anti-Olig2 IgG (Millipore, AB9610, 1:1,000); goat anti-Olig2 (R&D systems, 1:1,000); mouse anti-PCNA (Novocastra, PC-10, 1:100); rabbit anti-PSmad3(S423/S425) polyclonal antibody (Millipore 07-1389, 1:1,000); mouse anti-phospho-vimentin IgG (MBL, D076-3S, 1:1,000); goat anti-Prox1 IgG (R&D systems, 1:1,000); mouse anti-S100β (Sigma, 1:1,000); rabbit Sox2 antibody (Millipore, 1:1,000); goat anti-Sox2 IgG (R&D systems, 1:1,000); goat anti-Sox9 polyclonal antibody (R&D systems, 1:1,000).
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