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Lb agar powder

Manufactured by Sangon
Sourced in China

LB agar powder is a microbiology medium used for the cultivation and isolation of bacteria. It provides essential nutrients and solidifying agents necessary for the growth of a wide range of bacterial species.

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2 protocols using lb agar powder

1

Microbial Interaction Study Protocols

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Methylene blue (MB), 1-bromododecane, pyrene, methyl orange (MO), dodecyl trimethyl ammonium bromide (DTAB) and 9,10-diphenylanthracene (DPA) were purchased from Aladdin Regent Co., Ltd (Shanghai, China). In addition, LB broth powder and LB agar powder were purchased from Sangon Biotech (Shanghai, China). All other reagent were purchased from Aladdin Regent Co., Ltd (Shanghai, China) without further purification.
P. aeruginosa YM4 (CCTCC No. M2017494) was provided by Prof. Shuang Li, Nanjing Tech University. S. aureus (CICC No. 21600) were kindly supplied by Dr Lixian Xu, the second affiliated hospital of Nanjing Medical University.
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2

Culturing Cell Lines and Bacterial Strains

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LX-2, HT-29, and THP-1 cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA). Cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco), 100 U/mL penicillin, and 100 mg/mL streptomycin. C29 was purchased from Med Chem Express (NJ, USA). In this study, the most common clinical strain of Enterococcus was used (40 (link), 41 (link)). Enterococcus faecalis was grown in LB agar powder (Sango Biotech) in a shaker at 37°C for 8 h.
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