Pcf q260azi

Manufactured by Olympus

Sourced in Japan

The PCF-Q260AZI is a compact, high-performance fluorescence microscope system designed for laboratory applications. It features a high-intensity LED light source and a sensitive camera for capturing detailed fluorescence images. The system is equipped with a range of filter sets to accommodate various fluorescence labeling techniques. The core function of the PCF-Q260AZI is to provide researchers with a versatile and reliable tool for fluorescence imaging and analysis in a laboratory setting.

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Lab products found in correlation

Cf h260azi, by Olympus (13 mentions) Pcf h290zi, by Olympus (8 mentions) Cf hq290i, by Olympus (7 mentions) Gif q260j, by Olympus (7 mentions) Cf hq290zi, by Olympus (7 mentions) Pcf q240zi, by Olympus (6 mentions) Pcf q260ji, by Olympus (6 mentions) Hx 610 135, by Olympus (6 mentions) Pcf h290i, by Olympus (4 mentions) Evis lucera elite, by Olympus (4 mentions) Pcf h290ti, by Olympus (4 mentions) Esg 100, by Olympus (4 mentions) Dualknife, by Olympus (3 mentions) D 201 12704, by Olympus (3 mentions) Md 48710 evl device, by Sumitomo Bakelite (3 mentions) Coagrasper, by Olympus (3 mentions) Mucoup, by Johnson & Johnson (3 mentions) Cf q260ai, by Olympus (3 mentions) Vio300d, by Erbe (3 mentions) Ec l600zp, by Fujifilm (2 mentions)

42 protocols using pcf q260azi

Colonoscopy Equipment Evaluation and Treatment

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In the retroflexion ability analysis, the CF-H260AZI, PCF-Q260AZI, or CF-HQ290I (Olympus) was used in the latest evaluating colonoscopy. PCF-Y0062 is the prototype of the novel PCF-H290TI colonoscope. As our institution had only one, it was impossible to use it for all cases during the study period, considering that there were so many treatments being performed simultaneously, and the scope required adequate cleaning time. Therefore, the PCF-Y0062 was preferentially used on lesions for which some difficulties were anticipated, such as poor endoscope operability identified during the evaluating colonoscopy. A conventional gastroscope, GIF-Q260 J, GIF-H260Z, or GIF-H260 (Olympus), was used for some lesions located in the sigmoid colon and rectum that required retroflexion, and a conventional colonoscope, PCF-Q260AZI, or CF-H260AZI (Olympus), was used for the other lesions.
In the treatment outcome analysis, the conventional colonoscopes included the PCF-Y0021 (prototype, Olympus), PCF-Y0047 (prototype, Olympus), PCF-Q260AZI, CF-H260AZI, and PCF-Q260JI (Olympus).

Tanaka H., Oka S., Tanaka S., Inagaki K., Okamoto Y., Matsumoto K., Boda K., Yamashita K., Sumimoto K., Ninomiya Y., Hayashi N, & Chayama K. (2019). The utility of a novel colonoscope with retroflexion for colorectal endoscopic submucosal dissection. Endoscopy International Open, 7(2), E130-E137.

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Endoscopic Resection Techniques for Colorectal Lesions

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Figure 1 shows the procedure for each ER method. ER was conducted by experienced endoscopists. cEMR was performed using a single‐channel colonoscope (PCF‐Q260AZI or PCF‐H290ZI; Olympus). After injecting 10% glycerin solution mixed with a small amount of indigo‐carmine to lift the lesion, the base of the lesion was captured with a snare (SnareMaster; Olympus) and cut using an electrosurgical current. ESD was performed using a single‐channel colonoscope (PCF‐Q260AZI or PCF‐H290TI; Olympus) with DualKnife or DualKnife‐J (Olympus). After injecting 10% glycerin solution and/or 0.4% sodium hyaluronate solution (MucoUp; Johnson & Johnson, New Brunswick, NJ, USA) mixed with a small amount of indigo carmine into the submucosal layer, a circumferential incision was made, and submucosal dissection was then performed. ESMR‐L was conducted using a ligation device (Sumitomo Bakelite Co. Ltd., Tokyo, Japan) attached to a single‐channel colonoscope (PCF‐Q260AZI or PCF‐H290ZI). After injecting 10% glycerin solution mixed with a small amount of indigo carmine into the submucosal layer, the lifted lesion was suctioned into the ligation device, and an elastic band was deployed.¹⁹ The lesion was resected by snaring under the band.

Kamigaichi Y., Yamashita K., Oka S., Tamari H., Shimohara Y., Nishimura T., Inagaki K., Okamoto Y., Tanaka H., Yuge R., Urabe Y., Arihiro K, & Tanaka S. (2021). Clinical outcomes of endoscopic resection for rectal neuroendocrine tumors: Advantages of endoscopic submucosal resection with a ligation device compared to conventional EMR and ESD. DEN Open, 2(1), e35.

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Colonoscopy Preparation and Procedure

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Colonoscopy, including early colonoscopy, is performed after preparation with 2 L of polyethylene glycol (PEG). If patients have difficulty ingesting PEG due to poor general condition, either high-pressure enema or no preparation was carried out before colonoscopy. Carbon dioxide insufflation was used in all patients to reduce abdominal discomfort except for those with chronic obstructive pulmonary disease. PCF-Q260AZI (Olympus, Tokyo, Japan), which has a water jet system, was used for early colonoscopy with a cap attachment and PCF-Q260AZI or CF-H260AZI (Olympus, Tokyo, Japan) for elective colonoscopy.

Doi H., Sasajima K., Takahashi M., Sato T., Ootsu I, & Chinzei R. (2020). Effectiveness of Conservative Treatment without Early Colonoscopy in Patients with Colonic Diverticular Hemorrhage. Canadian Journal of Gastroenterology & Hepatology, 2020, 3283940.

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Colonoscopic Image Dataset for Deep Learning

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This study was approved by the Ethics Committee of the National Cancer Center, Tokyo, Japan. All methods were performed in accordance with the Ethical Guidelines for Medical and Health Research Involving Human Subjects. Informed consent was obtained from each patient included in the study. All colonoscopic still and video images were obtained at this institution. We retrospectively collected images of colonoscopies performed between January 2015 and June 2016. The images were assigned to the training set of the deep learning model (obtained between January 2015 and April 2016) or the validation set (obtained between May 2016 and June 2016). All images were obtained using standard endoscopes (PCF-Q240ZI, CF-H260AZI, PCF-Q260AZI, CF-HQ290AI, or PCF-H290AZI; Olympus Optical Co., Tokyo, Japan and EC-580RD/M, EC-590MP, EC-590ZP, EC-590WM3, EC-600ZW/M, EC-600WM, EC-L600ZP; Fujifilm Medical Co., Tokyo, Japan) and a standard video processor system (EVIS LUCERA system; Olympus Optical; Advancia HD or LASEREO system; Fujifilm Medical).

Yamada M., Saito Y., Imaoka H., Saiko M., Yamada S., Kondo H., Takamaru H., Sakamoto T., Sese J., Kuchiba A., Shibata T, & Hamamoto R. (2019). Development of a real-time endoscopic image diagnosis support system using deep learning technology in colonoscopy. Scientific Reports, 9, 14465.

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Colonoscopy Techniques for Diverticular Bleeding

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When the patient's general condition was stable, colonoscopy was performed after preparation with colonic lavage (complete ingestion of 2 L polyethylene glycol [PEG]-based lavage solution) over 2-4 h. A standard video colonoscope (PCF-Q260I, PCF-Q260AZI; Olympus Optical, Tokyo, Japan) or a colonoscope with a water-jet function was used (PCF-Q260JI) for all procedures (Figure 1A). A transparent cap (D201-12704, MG-163; Olympus Optical) was attached on the tip of the endoscope (Figure 1B). An expert colonoscopist was characterized as having conducted >1000 colonoscopies[10 ]. We also used an NT tube (Olympus Optical) for the removal of intradiverticular feces and blood clots to detect an exposed vessel, washing each diverticulum as much as possible (Figure 1C-E, 2A-D). The transparent cap, water-jet scope, and NT tube were used according to the physician's preferences throughout the study period. A water-jet scope has been used for CDB since 2010, and an NT tube has been used since 2006.

Sato Y., Nakatsu-Inaba S., Matsuo Y., Yamashita M., Ikeda H., Yasuda H, & Itoh F. (2021). Efficient Colonoscopic Identification of Colonic Bleeding Diverticulum Using Intradiverticular Water Injection with a Nontraumatic Tube. Journal of the Anus, Rectum and Colon, 5(3), 313-318.

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Colonoscopy with EVIS LUCERA Systems

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All procedures were performed with EVIS LUCERA SPECTRUM and EVIS LUCERA ELITE systems (Olympus, Optical Co., Ltd., Tokyo, Japan) and colonoscopes CF-H260AZI, PCF-Q260AZI, and PCF-Q240ZI (Olympus Optical Co., Tokyo, Japan). The NBI settings were fixed at a surface structure enhancement level of A-8 with an adaptive hemoglobin color index enhancement level of 3.

Nakamura H., Ikematsu H., Osera S., Ito R., Sato D., Minamide T., Okamoto N., Yamamoto Y., Hombu T., Takashima K., Nakajo K., Kadota T., Yoda Y., Hori K., Oono Y, & Yano T. (2017). Visual assessment of colorectal flat and depressed lesions by using narrow band imaging. Endoscopy International Open, 5(12), E1284-E1288.

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Colorectal Neoplasia Detection via NBI

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We retrospectively searched for colorectal NBI images at the Sixth Affiliated Hospital, Sun Yat-sen University, and Guangdong Second Provincial Central Hospital. These images were used to train the model and have internal validation tests. The scopes used in this study were PCF-Q260JI, CF-H260AI, PCF-Q260AZI, PCF-Q260A3I, CF-H290I, CF-HQ290I, PCF-H290ZI, and CF-HQ290Z (Olympus Optical Co., Ltd., Tokyo, Japan), and the machines we used were CV-260 and CV-290 (Olympus Medical Systems). The collected images met the following criteria: NBI images; with pathology (if the image was normal mucosa, then pathology was not necessary); the pathology was made by the whole resection specimens rather than biopsy; good quality; and repeated or blurred images were excluded. Additionally, we collected the following information of the patients: age, sex, and information of the polyps: location, size, number, and pathology. The collected images were categorized into 4 types according to their pathology: type 1, hyperplastic or inflammatory polyps; type 2, adenomatous polyps, intramucosal cancer, or superficial submucosal invasive cancer; type 3, deep submucosal invasive cancer; and type 4, normal mucosa.

Lu Y., Wu J., Zhuo X., Hu M., Chen Y., Luo Y., Feng Y., Zhi M., Li C, & Sun J. (2022). Real-Time Artificial Intelligence-Based Histologic Classifications of Colorectal Polyps Using Narrow-Band Imaging. Frontiers in Oncology, 12, 879239.

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Colorectal Polyp Diagnosis via NBI-ME

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All endoscopists participated in an intensive, 1-hour, interactive training program on endoscopic diagnosis of colorectal polyp including diagnosis of NBI with magnification by an expert endoscopist (T. U.) before performing CP. Patients underwent bowel preparation consisting of sodium picosulfate the day before colonoscopy and 2 to 3 L of polyethylene glycol solution the morning before the procedure. Colonoscopies were exclusively performed using a colonoscope with magnification capability and water irrigation function (PCF-Q260AZI or PCF-H290AZI, Olympus Co., Tokyo, Japan). After cecal intubation, all detected polyps were photographed, and their characteristics, including size, location, macroscopic type and findings of magnified endoscopy with NBI-ME, were documented. Lesion size was estimated according to comparison to endoscopic devices. Cessation of antithrombotic drugs was done in accordance with guidelines for gastroenterological endoscopy in patients undergoing antithrombotic treatment published by the Japan Gastroenterological Endoscopy Society in 2014
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. Procedures were performed by three experts who had performed more than 5000 colonoscopies, four gastroenterologists who had performed 1000 to 4999 colonoscopies and six trainees who had performed fewer than 1000 colonoscopies.

Kato M., Abe K., Kubosawa Y., Sunata Y., Hirai Y., Hirata T., Takada Y., Wada M., Takatori Y., Banno S., Kinoshita S., Mori H., Takabayashi K., Kikuchi M., Shiraishi J, & Uraoka T. (2018). Validation of treatment algorithm based on the Japan narrow-band imaging expert team classification for sub-centimeter colorectal polyps. Endoscopy International Open, 6(8), E934-E940.

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Hemostatic Endoscopic Submucosal Injection and Clipping for Diverticular Bleeding

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The procedure was performed by various endoscopists including non-experts; however, all cases were performed under expert supervision to ensure quality of practice. In those undergoing HSE-C, once the source of active bleeding is identified, between 0.5 mL and 2.0 mL of HSE solution is injected into the submucosa around the neck of the responsible diverticulum, a process which is repeated 1–4 times (Figure 2). Injections are generally repeated until the bleeding is weakened enough to gain an improved visual field, so that clipping can be performed in a stable environment. HSE-C injection was abbreviated in the monotherapy group. The clipping method administered was either a direct method, where a clip is placed directly into the diverticulum and onto the bleeding vessel, or an indirect method, where the opening of the bleeding diverticulum is indirectly closed off via a zipper method (Figure 3). Direct clipping is generally the method of choice, with indirect clipping only performed when visibility is insufficient or if the maneuverability of the scope is poor. Colonoscopy was performed using one of the following scopes (PCF-Q260AZI, PCF-H290ZI, PCF-H290I, CF-HQ290I, Olympus Medical Systems, Tokyo, Japan), fitted with a transparent hood, and EZ clips (Olympus Medical Systems) were used for clipping.

Hamada S., Teramoto A., Zukeyama R., Matsukawa S., Fukuhara T., Takaki R., Utsumi T., Nakamura M., Kobashikawa K., Uchima N., Nakayoshi T, & Kinjo F. (2022). Efficacy of Combination Therapy with Epinephrine Local Injection and Hemostatic Clips on Active Diverticular Bleeding. Journal of Clinical Medicine, 11(17), 5195.

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Endoscopic Submucosal Dissection of Colorectal Lesions

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ESD was carried out with a PCF-Q240JI, PCF-Q260AZI or GIF-Q260J endoscope (Olympus Medical Systems Corp., Tokyo, Japan) equipped with a short type small caliber-tip transparent hood (Fujifilm Corp., Tokyo, Japan) fitted to the tip of the endoscope to retract the SM layer, thereby facilitating dissection. The procedures were primarily performed using a bipolar needle knife (Xeon Medical Corp., Tokyo, Japan) and insulation-tipped knife (Olympus Medical Systems Corp., Tokyo, Japan). Midazolam (2 mg intravenously) and pentazocine hydrochloride (15 mg intravenously) were administered during all ESD procedures. An additional 2 mg midazolam was given as necessary whenever indicated based on the judgment of the colonoscopist. Bipolar hemostatic forceps (Pentax Corp., Tokyo, Japan) were used for hemostasis of bleeding. CO₂ insufflation was used instead of air insufflation to reduce patient discomfort. Lesion margins were delineated before ESD using 0.4 % indigo–carmine spray dye. After injection of 10 % glycerol and 5 % fructose in normal saline solution (glycerol; Chugai Pharmaceutical Corp., Tokyo, Japan) and sodium hyaluronate acid (Muco-up; Johnson & Johnson Corp., Tokyo, Japan) into the SM layer, a circumferential incision was made using a bipolar needle knife, and ESD was the performed using one or two ESD knives.

Nakamura F., Saito Y., Sakamoto T., Otake Y., Nakajima T., Yamamoto S., Murakami Y., Ishikawa H, & Matsuda T. (2014). Potential perioperative advantage of colorectal endoscopic submucosal dissection versus laparoscopy-assisted colectomy. Surgical Endoscopy, 29(3), 596-606.

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