Victor 5 multilabel reader

Manufactured by PerkinElmer

The Victor V multilabel reader is a versatile and reliable instrument designed for a wide range of microplate-based assays. It is capable of performing absorbance, fluorescence, and luminescence measurements, making it suitable for various applications in life science research, drug discovery, and clinical diagnostics.

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Lab products found in correlation

Wallac 1420 workstation, by PerkinElmer (2 mentions) Peroxidase conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Receptor destroying enzyme, by Denka Seiken (1 mentions) Verikine high sensitivity mouse ifn β elisa kit, by PBL Assay Science (1 mentions) Quickplex sq 120 imager, by MSD (1 mentions) Prism 9, by GraphPad (1 mentions) Delta rbd, by ACROBiosystems (1 mentions) 1 step ultra tmb elisa substrate, by Thermo Fisher Scientific (1 mentions)

3 protocols using victor 5 multilabel reader

Hemagglutination Inhibition Assay for Influenza

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Sera were pre-treated with receptor-destroying enzyme (Denka-Seiken, Tokyo, Japan) and were subjected to hemagglutinin (HA) inhibition (HAI) assays as described [29 (link),31 (link)]. Turkey erythrocytes (0.5%), guinea pig erythrocytes (0.75%) and horse erythrocytes (1%) were used in HAI assays for influenza H1N1, H3N2 and H5N1 viruses respectively [29 (link),31 (link)]. HA-specific IgG ELISA was performed in 96-well plates pre-coated with 0.5 µg/ml of H5 recombinant HA (rHA) (Protein Sciences, Meriden, CT), or with 0.2 µg/ml of H1 or H3 rHA (Immune Technology, New York, NY) [32 (link)]. Bound antibodies were detected with peroxidase-conjugated secondary antibodies (Life technologies) and optical density (OD) at 450 nm was measured using a Victor V multilabel reader (PerkinElmer, Waltham, MA).

Finch C.L., Zhang A., Kosikova M., Kawano T., Pasetti M.F., Ye Z., Ascher J.R, & Xie H. (2019). Pregnancy level of estradiol attenuated virus-specific humoral immune response in H5N1-infected female mice despite inducing anti-inflammatory protection. Emerging Microbes & Infections, 8(1), 1146-1156.

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Quantifying Cytokines and Interferons in Mouse Tissues

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Proinflammatory cytokines and chemokines in mouse lung and liver homogenates were determined using MSD mouse TH1/TH2 9-plex (Meso Scale Diagnostic #N05013B-1) or MSD prototype mouse 7-plex (Meso Scale Diagnostic #N75ZB-1) according to the manufacturer’s protocols and data were acquired in a MESO QuickPlex SQ 120 imager equipped with MSD Discovery Workbench 4.0.12 (LSR_4_0_12). Type I interferons (IFNs) in mouse lung and liver homogenates were quantitated using VeriKine High Sensitivity mouse IFN-α all subtype ELISA kit (PBL Assay Science #42115-1) or Verikine High Sensitivity mouse IFN-β ELISA kit (PBL Assay Science #42410-1). Optical density (OD) at 450 nm was measured using a Victor V multilabel reader (PerkinElmer) equipped with Wallac 1420 Workstation (Version 3 Revision 4).

Radvak P., Kwon H.J., Kosikova M., Ortega-Rodriguez U., Xiang R., Phue J.N., Shen R.F., Rozzelle J., Kapoor N., Rabara T., Fairman J, & Xie H. (2021). SARS-CoV-2 B.1.1.7 (alpha) and B.1.351 (beta) variants induce pathogenic patterns in K18-hACE2 transgenic mice distinct from early strains. Nature Communications, 12, 6559.

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SARS-CoV-2 RBD Protein Binding Assay

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The following His-tagged recombinant RBD proteins (SEC-MALS verified) were purchased from Acrobiosystems, including (1) original RBD (#SPD-C52H3), (2) Kappa RBD bearing L452R and E484Q (#SPD-C52Hv), and (3) Delta RBD bearing L452R and T478K (#SPD-C52Hh). Serially diluted human sera were added to 96-well microtiter plates pre-coated with 1 μg/mL of recombinant RBD protein and were incubated at room temperature for 1h. For avidity assays, the plates were washed and overlaid with 100 μL/well of 4 M urea for 15 min.⁷⁴ (link) The plates were washed and re-blocked in blocking buffer for another hour. Bound IgG was detected using peroxidase-conjugated goat anti-human IgG (H + L) (Seracare #5220-0330, 1:2000) followed by 1-Step Ultra TMB-ELISA substrate (ThermoFisher # 34028). Optical density (OD) at 450 nm was measured using a Victor V multilabel reader (PerkinElmer) equipped with Wallac 1420 Workstation (Version 3 Revision 4). Endpoint ELISA titers were calculated based on OD values > 2-fold of blank. Avidity index was calculated as the area under curve (AUC) using Prism 9.3.1 (GraphPad).

Kwon H.J., Kosikova M., Tang W., Ortega-Rodriguez U., Radvak P., Xiang R., Mercer K.E., Muskhelishvili L., Davis K., Ward J.M., Kosik I., Holly J., Kang I., Yewdell J.W., Plant E.P., Chen W.H., Shriver M.C., Barnes R.S., Pasetti M.F., Zhou B., Wentworth D.E, & Xie H. (2022). Enhanced virulence and waning vaccine-elicited antibodies account for breakthrough infections caused by SARS-CoV-2 delta and beyond. iScience, 25(12), 105507.

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