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2 protocols using rabbit igg antibodies to gpr32

1

Resolvin-Mediated Inhibition of Inflammatory Signaling

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Recombinant human or mouse IL-17A was purchased from R&D Systems. The D series resolvins, RvD1 and RvD2, and MK2206 (Akt inhibitor) were from Cayman Chemical. SB202190 (p38 MAP kinase inhibitor), SB216763 (GSK-3β inhibitor), SN50 (NF-κB inhibitor), SP600125 (JNK inhibitor) were obtained from EMD-Millipore. LY294002 (PI3K inhibitor) and LY30351 (inactive analog of LY294002) were purchased from Sigma-Aldrich. Rabbit polyclonal antibody to Del-1 was from ProteinTech. Rabbit monoclonal antibody (mAb) to β-actin (13E5), rabbit IgG antibodies to total Akt (phosphorylation state–independent), phospho-Akt (Ser473), total GSK-3β and phospho-GSK-3β (Ser9), as well as rabbit anti-phospho-C/EBPβ (Thr-188) and anti-phospho-threonine IgG antibodies were purchased from Cell Signaling Technology. Rabbit IgG antibodies to GPR32 and to ALX/FPR2 were from Abcam and Abnova, respectively. FITC- or PE-conjugated goat anti-rabbit IgG and rabbit IgG antibody to C/EBPβ were purchased from Santa Cruz Biotechnology. All reagents (including those mentioned in the description of assays below) were used at doses based on our previously published work or on experiments in this study (see Supplementary fig. 1 and 4).
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2

Resolution of Inflammation Pathways

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Recombinant human or mouse IL-17A was purchased from R&D Systems. The D series resolvins, RvD1 and RvD2, and MK2206 (Akt inhibitor) were from Cayman Chemical. SB202190 (p38 MAP kinase inhibitor), SB216763 (GSK-3β inhibitor), SN50 (NF-κB inhibitor), SP600125 (JNK inhibitor) were obtained from EMD-Millipore. LY294002 (PI3K inhibitor) and LY30351 (inactive analogue of LY294002) were purchased from Sigma-Aldrich. Rabbit polyclonal antibody to Del-1 was from ProteinTech. Rabbit monoclonal antibody (mAb) to β-actin (13E5), rabbit IgG antibodies to total Akt (phosphorylation state independent), phospho-Akt (Ser473), total GSK-3β and phospho-GSK-3β (Ser9), as well as rabbit anti-phospho-C/EBPβ (Thr-188) and anti-phospho-threonine IgG antibodies were purchased from Cell Signaling Technology. Rabbit IgG antibodies to GPR32 and to ALX/FPR2 were from Abcam and Abnova, respectively. FITC- or PE-conjugated goat anti-rabbit IgG and rabbit IgG antibody to C/EBPβ were purchased from Santa Cruz Biotechnology. All reagents (including those mentioned in the description of assays below) were used at doses based on our previously published work or on experiments in this study (see Supplementary Figs 1 and 4).
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