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Alphatrak2 test 2 strips

Manufactured by Abbott

The AlphaTrak2 test 2 strips are a diagnostic tool used to measure glucose levels in cats and dogs. The strips provide a quick and convenient method for pet owners to monitor their pet's blood glucose levels at home.

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2 protocols using alphatrak2 test 2 strips

1

Metabolic Profiling in Aged Mice

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At around 13–14 weeks of age, tissues and blood were collected for metabolic and biochemical profiling from a separate cohort of mice. Blood was collected by cardiac puncture under terminal anaesthesia into Microtube 1.1 mL Z-Gel (Starstedt AG & Co), centrifuged at 10,000 rpm for 10 min at 4 °C and stored at − 80 °C until use. Plasma Serum was submitted and analysed in the Core Biochemical Assay Laboratory (CBAL). Mouse insulin and leptin were measured using a Meso Scale Discovery two-plex mouse metabolic immunoassay kit according to the manufacturer’s instructions and using calibrators provided by Meso Scale Diagnostics. Blood glucose was measured using approximately 2 μl blood drops using a glucometer (AlphaTrak2; Abbot Laboratories) and glucose strips (AlphaTrak2 test 2 strips, Abbot Laboratories, Zoetis).
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2

Metformin Effects on Insulin Sensitivity in Mice

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Mice generation and protocol as Study 3, except aged 4 to 6 months.
On day of first gavage body weights (mean±SEM) of study groups were
35.1±1.2g; 35.05±1.2g for wild type Vehicle and Metformin
treatment respectively, and 35.08±1.02g; 35.02±1.47g for
Gdf15-/- Vehicle and Metformin treatment
respectively. On day 11, after final dose of metformin mice were fasted for
4 hours. Baseline venous blood sample was collected into heparinised
capillary tube for insulin measurement and blood glucose was measured using
approximately 2 μl blood drops using a glucometer (AlphaTrak2; Abbot
Laboratories) and glucose strips (AlphaTrak2 test 2 strips, Abbot
Laboratories, Zoetis). Mice were given intraperitoneal injection of insulin
(0.5U/kg mouse, Actrapid, NovoNordisk Ltd) and serial mouse glucose levels
measured at time points indicated. Mice were sacrificed by terminal
anaesthesia as in Study 2. Mouse insulin was measured using a 2-plex Mouse
Metabolic immunoassay kit from Meso Scale Discovery Kit (Rockville, MD,
USA), performed according to the manufacturer’s instructions and
using calibrators provided by MSD. Serum metformin levels were quantified
using a stable isotope dilution LC-MS/MS method described
previously33 (link).
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