Carboxyfluorescein succinimidyl ester (cfse)
CFSE is a fluorescent dye used for cell proliferation assays. It binds to cellular proteins, allowing for the tracking of cell division by flow cytometry.
Lab products found in correlation
7 protocols using carboxyfluorescein succinimidyl ester (cfse)
Neutrophil Adhesion Assay: ICAM-1 and CXCL1
Murine Dendritic Cells Regulate CD4+ T Cell Activation
bone-marrow-derived dendritic cells (BMDCs) (5 × 105 cells/mL) from BALB/c mice were pretreated with the vehicle
or broad Ca2+ channel blocker (SKF96365, 20 μM) and
treated with vehicle
in combination with MPLA (100 ng/mL) for 24 h. BMDCs were loaded with
the OVA protein (10 μg/mL) for 4 h, washed twice, and co-cultured
with the same number of CFSE-labeled CD4 T cells from spleens of sex-matched
DO11.10 TCR transgenic mice for 72 h. CD4+ T cells were
isolated from spleens of DO11.10 TCR transgenic mice using an EasySep
Mouse CD4 T cell isolation kit (STEMCELL Technologies, Vancouver,
Canada) and labeled with CFSE (4 μM, Molecular Probe, Eugene,
OR, United States). Supernatants were assayed for IFN-γ and
IL-2 by ELISA, and cell suspensions were subjected to fluorescence-activated
cell sorting (FACS) analysis of CFSE dilution of DO11.10 CD4 T cells.
T cell division was analyzed by a MACSQuant Analyzer 10 (Miltenyi
Biotec, Bergisch Gladbach, Germany) using AF647-conjugated anti-DO11.10
TCR antibodies (eBioscience, San Diego, CA, United States). The %
divided, the percent of the live CFSE-labeled CD4+ T cells
that entered division, was calculated using FlowJo (version 10.6.1,
Becton Dickinson, Ashland, OR, United States).
CFSE-Based Lymphocyte Proliferation Assay
CFSE-Labeled T Cell Proliferation Assay
Neutrophil-T Cell Coculture Assay
Quantifying Cell Internalization by Flow Cytometry
CFSE-based Lymphocyte Proliferation Assay
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