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Human il 18 platinum elisa

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The Human IL-18 Platinum ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-18 (IL-18) in serum, plasma, and cell culture supernatants.

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Lab products found in correlation

Mouse il 18 platinum elisa, by Thermo Fisher Scientific (1 mentions) Human tnfα elisa ready set go, by Thermo Fisher Scientific (1 mentions) Spectamax m5, by Molecular Devices (1 mentions) Human il 1β elisa, by Thermo Fisher Scientific (1 mentions) Model 450 microplate reader, by Bio-Rad (1 mentions) Human tim 1 kim 1 havcr quantikine elisa kit, by R&D Systems (1 mentions) Bms 267 2, by Thermo Fisher Scientific (1 mentions) Cytometric bead array cba, by BD (1 mentions)

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Human IL-6 and IL-18 Platinum ELISA kits Human IL-18 Platinum ELISA Kit IL-18 ELISA IL-18 ELISAs

7 protocols using human il 18 platinum elisa

1

Quantifying IL-18 and IL-18BP in Plasma

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IL-18 and IL-18BP plasma levels were measured using ELISA kits (mouse IL-18 Platinum ELISA, BMS618/2, human IL-18 Platinum ELISA, BMS267/2CE; eBioscience and human IL-18BP ELISA Kit, ab100559; Abcam, Paris, France) following the manufacturer's instructions. Free IL-18 levels were calculated using IL-18 and IL-18BP levels as shown in Novick et al, (2001 (link)).
Uzan B., Poglio S., Gerby B., Wu C.L., Gross J., Armstrong F., Calvo J., Cahu X., Deswarte C., Dumont F., Passaro D., Besnard-Guérin C., Leblanc T., Baruchel A., Landman-Parker J., Ballerini P., Baud V., Ghysdael J., Baleydier F., Porteu F, & Pflumio F. (2014). Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression. EMBO Molecular Medicine, 6(6), 821-834.
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2

Quantification of Inflammatory Cytokines

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Human IL-18, IL-1β, and TNF-α were measured with the Human IL-18 Platinum ELISA, Human IL-1β ELISA and Human TNF-α ELISA Ready-SET-Go kits respectively (eBiosciences, San Diego CA) using 50 µL of plasma or 100 µL of culture supernatant. Each sample was tested in duplicate. Data was acquired using a SpectaMax M5 (Molecular Devices, Sunnyvale CA). The LLOD of IL-18 was 40 pg/mL in plasma and 18 pg/mL in culture supernatant.
Chattergoon M.A., Latanich R., Quinn J., Winter M.E., Buckheit RW 3.r.d., Blankson J.N., Pardoll D, & Cox A.L. (2014). HIV and HCV Activate the Inflammasome in Monocytes and Macrophages via Endosomal Toll-Like Receptors without Induction of Type 1 Interferon. PLoS Pathogens, 10(5), e1004082.
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3

Urinary Biomarkers for Acute Kidney Injury

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Clean-catch midstream urine specimens were collected from patients in each group at the time of admission and were collected again 48 h after admission. Patients with compromised mental status were given an indwelling catheter to obtain clean urine. After centrifugation for 5 min at 3,000 rpm, the supernatant was transferred into Eppendorf tubes and stored in a freezer at −80 °C. Urine specimens for the control group were also collected and stored at −80 °C.
Urinary KIM-1 and IL-18 levels were measured by enzyme-linked immunosorbent assay (ELISA) (Human TIM-1/KIM-1/HAVCR Quantikine ELISA Kit, R&D Systems, Minneapolis, MNUSA; Human IL-18 Platinum ELISA, eBioscience, Inc, San Diego, CA, USA). Optical density was read at 450 nm (Model 450 Microplate Reader; Bio-Rad Laboratories, Inc., Hercules, CA, USA), and the test-sample concentrations were calculated using a standard curve.
Ren H., Zhou X., Dai D., Liu X., Wang L., Zhou Y., Luo X, & Cai Q. (2015). Assessment of urinary kidney injury molecule-1 and interleukin-18 in the early post-burn period to predict acute kidney injury for various degrees of burn injury. BMC Nephrology, 16, 142.
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4

Quantifying IL-18 in Plasma and PBMC

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IL-18 was quantified in human plasma samples and in supernatants from E. coli-stimulated PBMC using the Human IL-18 Platinum ELISA (eBioscience, San Diego, CA).
Bolte F.J., O’Keefe A.C., Webb L.M., Serti E., Rivera E., Liang T.J., Ghany M, & Rehermann B. (2017). Intra-hepatic Depletion of Mucosal Associated Invariant T cells in Hepatitis C Virus-induced Liver Inflammation. Gastroenterology, 153(5), 1392-1403.e2.
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5

Cytokine Quantification by ELISA

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The concentration of IL-18 and IL-1β in cell supernatants was determined by ELISA using commercial quantitative kits according to the manufacturer’s instructions (#VAL101, Valukine™ ELISA Kit for human IL-1β; R&D, USA) (#BMS267-2, Human IL-1 8 Platinum ELISA; eBioscience, USA). All samples were analyzed in duplicate. Standard curves were generated during assays by plotting absorbance value against the gradient concentration of the standards provided with the kits. Both positive and blank controls were analyzed simultaneously on the same plate.
Guo Q., Wu Y., Hou Y., Liu Y., Liu T., Zhang H., Fan C., Guan H., Li Y., Shan Z, & Teng W. (2018). Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Frontiers in Immunology, 9, 1197.
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6

ELISA Measurements of Kidney Biomarkers

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We used commercially available enzyme-linked immunosorbent assay kits to measure concentrations of serum and urinary monomeric SNGAL and uNGAL (Human NGAL ELISA Kit, Catalog Number: KIT 036CE, BioPorto Diagnostic, Hellerup, Denmark), uKIM-1 (Human KIM-1 ELISA Test Kit, Catalog Number: H-RENA-E-001, BioAssay Works, Tyler CT, Ijamsville, MD, USA), and uIL-18 (Human IL-18 Platinum ELISA, Catalog Number: BMS267/2CE, Affymetrix, Santa Clara, CA, USA), with intra-assay and inter-assay coefficients of variability: 10.5% and 7.5% for NGAL, <10% and <10% for KIM-1, and 6.5% and 8.1% for IL-18, respectively. All measurements were performed in the laboratory of the Department of Pathophysiology, Medical Faculty in Katowice, Medical University of Silesia in Katowice. Quality control was assessed based on repeated measurements and is reported as average deviation (4.4% for NGAL; 7.1% for KIM-1, and 8.7% for IL-18).
Szumilas D., Owczarek A.J., Brzozowska A., Niemir Z.I., Olszanecka-Glinianowicz M, & Chudek J. (2024). The Value of Urinary NGAL, KIM-1, and IL-18 Measurements in the Early Detection of Kidney Injury in Oncologic Patients Treated with Cisplatin-Based Chemotherapy. International Journal of Molecular Sciences, 25(2), 1074.
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7

Cytokine Profiling in Serum

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IL-1 receptor antagonist (IL-1ra) and IL-8 levels in serum were measured by kinetic sandwich ELISA (Peprotech, Rocky Hill, NJ, USA). Serum levels of IL-1β, IL-6 and IL-10 were measured using Cytometric Bead Array (CBA) (BD Biosciences, San Jose, CA). IL-18 serum levels were measured using Human IL-18 Platinum ELISA (Affymetrix/eBioscience/ThermoFisher Scientific); IL-18 Binding Protein (BP) was measured using IL-18BP ELISA from Abcam (Cambridge, MA).
Zhang Y., Gupta S., Ilstad-Minnihan A., Ayyangar S., Hay A.D., Pascual V., Ilowite N.T., Macaubas C, & Mellins E.D. (2018). Interleukin-1 in Monocyte Activation Phenotypes in Systemic Juvenile Idiopathic Arthritis: observations from a clinical trial of rilonacept, an interleukin-1 inhibitor. Clinical immunology (Orlando, Fla.), 194, 9-18.
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