1 2 4 5 benzenetetraamine tetrahydrochloride y15 c6h10n4 4clh

Manufactured by Merck Group

1,2,4,5-benzenetetraamine tetrahydrochloride (Y15) (C6H10N4·4ClH) is a chemical compound used as a laboratory reagent. It has the chemical formula C6H10N4·4ClH. This compound is a crystalline solid that is soluble in water and other polar solvents.

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Lab products found in correlation

Anti c src sc 18, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3 9662, by Cell Signaling Technology (1 mentions) Pf 573 228 c22h20f3n5o3s, by Pfizer (1 mentions) Anti gapdh, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Mouse monoclonal anti β actin, by Merck Group (1 mentions) Mouse monoclonal anti β actin a1978, by Merck Group (1 mentions)

3 protocols using 1 2 4 5 benzenetetraamine tetrahydrochloride y15 c6h10n4 4clh

Immunoblotting Antibodies for Focal Adhesion Kinase

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Monoclonal mouse anti-FAK (4.47, 05-0537) and anti-phospho Src (Tyr 416, 05-677) antibodies were obtained from Millipore (EMD Millipore, Billerica, MA) and rabbit polyclonal anti-phospho-FAK (Y397, 71-7900) and anti-phospho Erk 1/2 (05-797R) antibodies were from Invitrogen (Invitrogen Corp., Carlsbad, CA). Rabbit polyclonal anti-PARP (9542S), anti-Akt (9272), anti-phospho Akt (Ser 473, 9271), anti-Erk 1/2 (9102) and anti-cleaved caspase 3 (9662) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Monoclonal mouse anti-Jnk (F-3, sc-1648), anti-phospho Jnk (G-7, sc-6254) and rabbit polyclonal anti-c-Src (sc-18) were from Santa Cruz Biotechnology (Santa Cruz, CA). Monoclonal mouse anti-GAPDH was from Millipore (MAB374) and anti-β-actin was from Sigma (A1978, Sigma-Aldrich Corp., St. Louis, MO). The small molecule PF-573,228 (C₂₂H₂₀F₃N₅O₃S) was obtained from Pfizer (New York, NY), and the small molecule 1,2,4,5-benzenetetraamine tetrahydrochloride (Y15) (C₆H₁₀N₄·4ClH) from Sigma.

Megison M.L., Gillory L.A., Stewart J.E., Nabers H.C., Mrozcek-Musulman E, & Beierle E.A. (2014). Inhibition of Focal Adhesion Kinase (FAK) Leads to Abrogation of the Malignant Phenotype in Aggressive Pediatric Renal Malignancies. Molecular cancer research : MCR, 12(4), 514-526.

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Antibody-Based FAK Signaling Assay

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Monoclonal mouse anti-FAK (4.47, 05-0537) antibody was from Millipore (EMD Millipore, Billerica, MA), and rabbit polyclonal anti-phospho-FAK (Y397, 71-7900) antibody was from Invitrogen (Invitrogen Corp., Carlsbad, CA). Rabbit polyclonal anti-cleaved PARP (9542) and rabbit monoclonal anti-PARP (9532) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Monoclonal mouse anti-β-actin was from Sigma (A1978; Sigma-Aldrich Corp., St. Louis, MO). The small molecule PF-573,228 (C₂₂H₂₀F₃N₅O₃S) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA), and the small molecule 1,2,4,5-benzenetetraamine tetrahydrochloride (Y15) (C₆H₁₀N₄∙ 4ClH) from Sigma.

Waters A.M., Stafman L.L., Garner E.F., Mruthyunjayappa S., Stewart J.E., Mroczek-Musulman E, & Beierle E.A. (2016). Targeting Focal Adhesion Kinase Suppresses the Malignant Phenotype in Rhabdomyosarcoma Cells. Translational Oncology, 9(4), 263-273.

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Western Blot Analysis of FAK and Related Signaling Proteins

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Monoclonal rabbit anti-phospho-FAK (Tyr 397, 700255) was obtained from Invitrogen (Rockford, IL). Polyclonal rabbit anti-FAK (C-20) was obtained from Santa Cruz Biotechnology (Dallas, TX). Polyclonal rabbit anti-cleaved PARP (AB3565) was obtained from EMD Millipore (Billerica, MA). Polyclonal rabbit anti-cleaved caspase 3 (9661S) was obtained from Cell Signaling Technology (Danvers, MA). Monoclonal rabbit anti-PDGFRβ (32570) was obtained from Abcam (Cambridge, MA). Monoclonal mouse anti-β-actin (A1978) was obtained from Sigma Aldrich (St. Louis, MO). The small molecule FAK inhibitor, PF-573,228 (PF;C₂₂H₂₀F₃N₅O₃S), was obtained from Santa Cruz. A second small molecule FAK inhibitor, 1,2,4,5-benzenetetraamine tetrahydrochloride (Y15; C₆H₁₀N₄·4ClH), was obtained from Sigma Aldrich. Sunitinib (C₂₂H₂₇FN₄O₂·C₄H₆O₅), a receptor tyrosine kinase inhibitor known to target PDGFRβ, was obtained from Selleckchem (Houston, TX).

Aye J.M., Stafman L.L., Williams A.P., Garner E.F., Stewart J.E., Anderson J.C., Mruthyunjayappa S., Waldrop M.G., Goolsby C.D., Markert H.R., Quinn C., Marayati R., Mroczek-Musulman E., Willey C.D., Yoon K.J., Whelan K.F, & Beierle E.A. (2019). The effects of focal adhesion kinase and platelet-derived growth factor receptor beta inhibition in a patient-derived xenograft model of primary and metastatic Wilms tumor. Oncotarget, 10(53), 5534-5548.

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