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Human glucagon

Manufactured by Merck Group
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Human glucagon is a lab equipment product produced by Merck Group. Glucagon is a hormone that helps regulate blood sugar levels by stimulating the liver to release stored glucose into the bloodstream.

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Lab products found in correlation

Sylgard 184, by Dow (1 mentions) Axio observer z1 inverted microscope, by Zeiss (1 mentions) Alexa fluor donkey anti mouse 488 nm, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Glucagon (128 citations) Mouse monoclonal anti-human glucagon (7 citations)

2 protocols using human glucagon

1

Graphene CVD on Annealed Copper

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Chemical vapor deposition graphene was grown on an annealed copper (Cu) foil (99.9%, 25 μm thick). Polydimethylsiloxane (PDMS, SYLGARD-184) was purchased from Dow Corning (Midland, MI) for the PDMS chamber. 1-Pyrenebutanoic acid succinimidyl ester (PASE), phosphate buffered saline (PBS), human insulin, human glucagon, and human somatostatin were purchased from Sigma-Aldrich (St. Louis, MO). Insulin-specific aptamer (IGA3, sequence 5′-NH2-GGT GGT GGG GGG GGT TGG TAG GGT GTC TTC-3′) was synthesized and purified by Integrated DNA Technologies (Coralville, IA).
Hao Z., Zhu Y., Wang X., Rotti P.G., DiMarco C., Tyler S.R., Zhao X., Engelhardt J.F., Hone J, & Lin Q. (2017). Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor. ACS applied materials & interfaces, 9(33), 27504-27511.
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2

Cryosectioning and Immunofluorescent Staining

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Explanted scaffolds were flash frozen in isopentane cooled on dry ice and then embedded within OCT embedding medium and cryosectioned. Sections were stained using human insulin (Dako) and human glucagon (Sigma, cat. no. G2654) primaries, followed by AlexaFluor donkey anti-mouse 488nm (Invitrogen, cat. no. A21202) and AlexaFluor 647nm AffiniPure donkey anti-guinea pig (Jackson Immuno Research Labs, cat. no. 706605148) secondaries. Fluoromount with DAPI was applied before placing coverslips. Digital images were acquired with a ZEISS Axio Observer Z1 inverted microscope.
Linos A., Berekaa M.M., Reichelt R., Keller U., Schmitt J., Flemming H.C., Kroppenstedt R.M, & Steinbüchel A. (2000). Biodegradation of cis-1,4-Polyisoprene Rubbers by Distinct Actinomycetes: Microbial Strategies and Detailed Surface Analysis. Applied and Environmental Microbiology, 66(4), 1639-1645.
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