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Ab19136

Manufactured by Abcam
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Ab19136 is a laboratory equipment product. It is designed for use in various scientific experiments and research applications.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Ab154374, by Abcam (1 mentions) Vector abc reagent, by Vector Laboratories (1 mentions) Fast dab peroxidase substrate, by Merck Group (1 mentions) Ab51502, by Abcam (1 mentions) Ab230261, by Abcam (1 mentions) Ab76442, by Abcam (1 mentions) Ab237703, by Abcam (1 mentions) Ab241060, by Abcam (1 mentions) Ab51252, by Abcam (1 mentions) Mab1568, by Merck Group (1 mentions) Sc 23948, by Santa Cruz Biotechnology (1 mentions) Homer1, by Synaptic Systems (1 mentions) Mab377, by Merck Group (1 mentions) Rab10, by Cell Signaling Technology (1 mentions) Ab5541, by Merck Group (1 mentions) Vamp2, by Synaptic Systems (1 mentions) Sc 58774, by Santa Cruz Biotechnology (1 mentions) α synuclein, by BD (1 mentions) Hre bla me 180, by Thermo Fisher Scientific (1 mentions)

5 protocols using ab19136

1

Immunohistochemical Localization of Heat Shock Proteins in Testis

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Immunohistochemistry was performed as previously described (Liu et al., 2017). Briefly, sections of P. sinensis testis were dewaxed in xylene, dehydrated in an ethanol series, and blocked with 3% H2O2 in distilled water. The sections were incubated with anti‐HSPA1L/HSPA1B‐L (ab154409, Abcam Inc., Cambridge, MA, USA; 1:100), anti‐HSPA2 (ab154374, Abcam Inc., Cambridge, MA, USA; 1:100), and anti‐Hsc70/HSPA8 (ab19136, Abcam Inc.; 1:150) antibodies separately overnight at 4℃. Negative controls were reacted with PBS instead of the specific antibodies. A biotinylated secondary antibody and Vector ABC reagent (Vector Laboratories) were subsequently added according to the manufacturer's instructions. Then, the sections were stained using the FAST DAB Peroxidase Substrate (Sigma) and counterstained with hematoxylin for 10 s. The slides were dehydrated and analyzed under a light microscope.
Liu T., Han Y., Liu Y, & Zhao H. (2019). Genomewide identification and analysis of heat‐shock proteins 70/110 to reveal their potential functions in Chinese soft‐shelled turtle Pelodiscus sinensis. Ecology and Evolution, 9(12), 6968-6985.
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2

Comprehensive Antibody Panel for Neuronal Characterization

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Rab10 (4262S, Cell Signaling Technology) for immunoblot, Rab10 (ab237703, Abcam) for immunofluorescence, pRab10 for immunoblot (ab230261, Abcam), pRab10 for immunofluorescence (ab241060, Abcam), Hsc70 (ab19136, Abcam), α-Tubulin (SC23948, Santa Cruz Biotechnology), parvalbumin, (NBP2-50036, NovusBio), NeuN (MAB377, Millipore), SATB2 (ab51502 Abcam), calretinin (MAB1568, Millipore), DARPP32 (MAB4230, R&D Systems), ChAT (NBP2-46620, NovusBio), DAT loop (6-8D6 Santa Cruz Biotechnology), tyrosine hydroxylase (TH) (ab76442, Abcam), CD68 (NBP2-33337SS, NovusBio), GFAP (AB5541, Millipore), Olig2 (MABN50 Millipore), KDEL receptor (sc-58774, Santa Cruz Biotechnology), TGN46 (MA3-063, ThermoFisher), LAMP1 (1D4B, DHSB), EEA1 (NBP2-36568, NovusBio), α-Synuclein (610786, BD Transduction Lab), Synuclein (ab51252, Abcam), VAMP2 (104 211, Synaptic Systems), Homer1 (160 006 Synaptic Systems), Rab8a (ab188574).
Singh V., Menard M.A., Serrano G.E., Beach T.G., Zhao H.T., Riley-DiPaolo A., Subrahmanian N., LaVoie M.J, & Volpicelli-Daley L.A. (2023). Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain. Acta Neuropathologica Communications, 11, 201.
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3

Regulation of Carbonic Anhydrase 9 in Hypoxic Cells

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Example 5

The human cervical cell line HRE-Bla ME-180 was obtained from Invitrogen. 6.2×105 cells were plated in 6-well plates (costar) in Opti-MEM plus 1% FBS (Invitrogen). 4 hrs later, cells were pretreated with chetomin, peptide or 0.5% DMSO (final concentration in media) vehicle control for 16 hrs under normoxia (21% O2); then incubation continued under normoxia or hypoxia (1% O2) for 24 hrs using a hypoxia incubator (Thermo Forma model). Total cell lysate form control, chetomin treated or SP-6 treated ME-180 cells were separated by SDS-PAGE and probed with anti-CA9 (1:2000, NB100-47, Novus biologicals) and anti HSC70 (1:5000, ab19136, Abcam). The results demonstrate that the protein level of Carbonic anhydrase 9 (CA9) was down-regulated by treating with SP-6 in ME-180 cells, as shown in FIG. 2.

US10246491B2. Peptidomimetic macrocycles and use thereof in regulating HIF1alpha (2019-04-02). AILERON THERAPEUTICS, INC. [US]. Inventors: Vincent Guerlavais [US], Noriyuki Kawahata [US], Huw M. Nash [US], Carl Elkin [US], Eric Feyfant [US].
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4

Indirect Immunofluorescence Staining Protocol

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For indirect immunofluorescence staining, cells grown on cover-slips were washed with PBS and fixed in cold acetone for 10 min at − 20 °C. Cells were then washed with PBS and incubated with primary antibodies diluted in PBS 1:100 of immunoaffinity-purified anti-NOA36 IgGs [5 (link)]; 1:500 of anti-FLAG M2 (SIGMA); 1:500 of anti-HA rabbit polyclonal antibody (ab9110 from AbCam) or 1:150 anti-HSPA8 rat monoclonal antibody (ab19136 from AbCam) at 37 °C for 45 min, and then washed with PBS for 30 min at room temperature (RT) and incubated with Alexa fluor 488 or 555-labeled secondary antibodies (Molecular Probes) at 37 °C for 45 min. Finally, cover-slips were washed twice in PBS and mounted in PBS-glycerol containing DAPI at 0.1 µg/mL. A Zeiss Axiophot microscope equipped with 10 ×, 20 × and 40 × NA objectives was routinely used. Images were taken using a SPOT Camera (Diagnostic Instruments Inc.) and processed using Adobe Photoshop software.
Sanchez-Briñas A., Duran-Ruiz C., Astola A., Arroyo M.M., Raposo F.G., Valle A, & Bolivar J. (2023). ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biology Direct, 18, 26.
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5

Proteomic Analysis Protocol

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OptiPrep was obtained from Accurate Chemical (Westbury, NY). EDTA-free Complete proteinase inhibitor was purchased from Roche (Indianapolis, IN). Criterion TGX gels, tris(2-carboxyethyl)phosphine hydrochloride, Precision Plus prestained molecular weight standards, 4X sample loading buffer and Clarity chemiluminescence reagent were obtained from Bio-Rad (Hercules, CA). Coomassie Plus Protein Assay Reagent and BCA Protein Assay kit were purchased from ThermoFisher (Waltham, MA). Autoradiography film was purchased from ISC Bioexpress (Kaysville, UT). Antibodies were purchased from the following sources: P-glycoprotein (sc-8313), nucleoporin (sc25523) and cathepsin D (sc377299) (Santa Cruz Biotechnology, Dallas, TX); GLUT-1 (ab32551), ATP synthase beta subunit (ab14730), Annexin 3 (ab127924), Annexin V (ab14196), Rab5 (ab13253), protein disulfide isomerase (ab2792), von Willebrand factor (ab6994), Hsc71 (ab19136), thioredoxin (ab86255) and PTRF (ab48824) from AbCam (Cambridge, MA); QSOX1 (12713-1-AP – Proteintech, Chicago, IL); caveolin-1 (610060 - BD Biosciences, San Jose, CA,); COX IV (4850) and Rab11a (2413) (Cell Signaling, Danvers, MA); and HRP-linked goat anti-rabbit and HRP-linked goat anti-mouse (GEHealthcare, Piscataway, NJ). All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless otherwise noted.
Tome M.E., Schaefer C.P., Jacobs L.M., Zhang Y., Herndon J.M., Matty F.O, & Davis T.P. (2015). Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. Journal of neurochemistry, 134(2), 200-210.
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