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Micropoint pulsed nitrogen

Manufactured by Lumencor
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The MicroPoint pulsed nitrogen is a specialized laboratory instrument that generates short, high-energy pulses of nitrogen gas. The core function of this product is to provide a controlled and precise source of nitrogen pulses for use in various scientific applications.

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Lab products found in correlation

Inverted axioobserver microscope, by Zeiss (2 mentions) 0.05 μm polystyrene microspheres, by Polysciences (2 mentions)

2 protocols using micropoint pulsed nitrogen

1

Exopher Photobleaching Dynamics Analysis

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Synchronized Is[Pmec-4mCh1] adult day 2 animals were immobilized on 7.5% M9 agarose pads with 2.5 μL PolySciences 0.05 μm polystyrene microspheres. Exopher centers were photo-bleached with 7 pulses of the MicroPoint pulsed nitrogen pumped dye laser (neutral density filter at position 9, Lumencor solid state light source) attached to a Zeiss Inverted Axio Observer microscope (100x 1.4 N.A. objective) on an anti-vibration table. 1 frame was recorded every 5 seconds using constant excitation intensity and exposure time with a Qimaging EXi Blue camera. Images were analyzed with ImageJ. Exopher fluorescence intensity was normalized to the intensity of the first data point in each series.
Melentijevic I., Toth M.L., Arnold M.L., Guasp R.J., Harinath G., Nguyen K.C., Taub D., Parker J.A., Neri C., Gabel C., Hall D.H, & Driscoll M. (2017). C. elegans Neurons Jettison Protein Aggregates and Mitochondria Under Neurotoxic Stress. Nature, 542(7641), 367-371.
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2

Exopher Photobleaching Dynamics Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Synchronized Is[Pmec-4mCh1] adult day 2 animals were immobilized on 7.5% M9 agarose pads with 2.5 μL PolySciences 0.05 μm polystyrene microspheres. Exopher centers were photo-bleached with 7 pulses of the MicroPoint pulsed nitrogen pumped dye laser (neutral density filter at position 9, Lumencor solid state light source) attached to a Zeiss Inverted Axio Observer microscope (100x 1.4 N.A. objective) on an anti-vibration table. 1 frame was recorded every 5 seconds using constant excitation intensity and exposure time with a Qimaging EXi Blue camera. Images were analyzed with ImageJ. Exopher fluorescence intensity was normalized to the intensity of the first data point in each series.
Melentijevic I., Toth M.L., Arnold M.L., Guasp R.J., Harinath G., Nguyen K.C., Taub D., Parker J.A., Neri C., Gabel C., Hall D.H, & Driscoll M. (2017). C. elegans Neurons Jettison Protein Aggregates and Mitochondria Under Neurotoxic Stress. Nature, 542(7641), 367-371.
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