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Water of lc ms grade

Manufactured by Thermo Fisher Scientific
Sourced in United States

Water of LC–MS grade is a high-purity water intended for use in liquid chromatography-mass spectrometry (LC-MS) applications. It is subjected to extensive purification processes to remove impurities and contaminants that could interfere with sensitive analytical techniques. This water meets the stringent quality standards required for reliable LC-MS analysis.

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3 protocols using water of lc ms grade

1

Quantification of Doping Agents in Urine

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The chemical
structures of the analytes are given in Figure 1. Analytical standards of DPG, DTG, and TPG
with purities ≥95% were purchased from Millipore-Sigma (St.
Louis, MO, USA). Isotopically labeled DPG (DPG-d10; purity
≥97%) was obtained from Toronto Research Chemicals (Toronto,
ON, Canada). Ammonium formate (97%; analytical grade) and formic acid
(88%; analytical grade) were purchased from Millipore-Sigma (St. Louis,
MO, USA). Methanol (MeOH) and water of LC–MS grade were obtained
from Fisher Scientific (Waltham, MA, USA). OasisⓇ hydrophilic–lipophilic
balanced (HLB) cartridges (60 mg/3 mL) were purchased from Waters
Corporation (Milford, MA, USA). Synthetic urine was purchased from
Cerilliant (Round Rock, TX, USA). Individual stock solutions of all
analytes and DPG-d10 were prepared in MeOH at 1 mg/mL.
Working solutions were diluted from stock solutions using MeOH.
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2

Standardized Analysis of Traditional Chinese Medicine

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Twenty-four authentic standards of adenosine (12), caffeic acid (17b), danshensu (17c), protocatechuic aldehyde (21), trans-5-caffeoylquinic acid (23b), ferulic acid (30), lithospermic acid (45b), salvianolic acid B (48a), baicalin (50a), ononin (50b), salvianolic acid A (61a), salvianolic acid C (70), astragaloside IV (77b), astragaloside III (79b), astragaloside II (83d), farrerol (84b), isoastragaloside II (85b), wogonin (86a), astragaloside I (92b), cyclosieversigenin (97b), dihydrotanshinone I (102a), tanshinone I (112), levistolide A (121) and tanshinone IIA (123) were all purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Five reference standards of calycosin7-O-β-d-glucoside (28b), rosmarinic acid (44), calycosin (62a), formononetin (80) and cryptotanshinone (130) were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The structures of these reference standards (purity > 98%) are shown in Fig. 2. They were stored at 4 °C before use.
Acetonitrile, formic acid and water of LC-MS grade were purchased from Fisher Scientific Company (USA). Methanol of analytical grade was obtained from Runjie Tech Co. (Shanghai, China).
DZT and its five ingredients, i.e., LC, SM, PA, AM, and HN were provided by Tonghua Baishan Pharmaceutical Co., Ltd. (Jilin, China).
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3

Mass Spectrometry Sample Preparation

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Methanol, acetonitrile, ethanol and water of LC–MS grade were purchased from Fisher Scientific (Loughborough, UK); 2.5-dihydroxybenzoic acid (DHB) and 9-aminoacridine hydrochloride monohydrate (9-AA) MALDI matrices were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium trifluoroacetate (NaTFA, Sigma-Aldrich) at 0.1 mg mL-1 (ACN/H2O 50:50 [v/v]) was used as a calibrant before each analysis.
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