Hirudin blood collection tube

Example 5

Blood was collected in a heparin blood collection tube manufactured by BD, and, 30 minutes later, a heparinase-BAPA mixed reagent was added to the blood such that their final concentrations were 0.17 IU/mL and 100 μM, respectively, to provide a blood sample.

For comparison, a blood sample collected in a hirudin blood collection tube (Roche Diagnostic) was used.

Using these blood samples, flow measurement of platelet thrombus formation was carried out using the device shown in FIG. 1. Blood was carried out at 18 μL/min.

In every case, a vacuum blood collection tube having a butyl rubber stopper was used.

The results are shown in FIG. 8.

By the addition of the heparinase-BAPA mixed reagent to the blood collected in the heparin blood collection tube, analysis of platelet thrombus formation was possible similarly to the case where blood was collected using the hirudin blood collection tube.

In contrast, when the blood collected in the heparin blood collection tube was directly subjected to similar measurement, a pressure increase due to platelet thrombus formation was hardly observed.

Example 4

Blood collected in a vacuum blood collection tube containing low-molecular-weight heparin (final concentration, 2 IU/mL) and CTI (final concentration, 50 μg/mL), in a hirudin blood collection tube (manufactured by Roche Diagnostic; hirudin final concentration, 50 μg/mL), or in a BAPA blood collection tube (BAPA final concentration, 100 μM) was used to measure platelet thrombus formation using the device shown in FIG. 1.

In every case, a vacuum blood collection tube having a butyl rubber stopper was used.

As a result, as shown in FIG. 7, the blood in each blood collection tube similarly allowed analysis of platelet thrombus formation. Also in a case where heparinase and BAPA reagent (final concentrations, 0.17 IU/mL and 100 μM, respectively) were added to the blood in the blood collection tube containing low-molecular-weight heparin and CTI 30 minutes after the blood collection, measurement of platelet thrombus formation was possible.