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Fls920

Manufactured by Hamamatsu Photonics
Sourced in United Kingdom

The FLS920 is a time-resolved fluorescence spectrometer manufactured by Hamamatsu Photonics. It is designed for the measurement of time-resolved fluorescence and phosphorescence signals.

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3 protocols using fls920

1

Characterizing Photosensitizers via Spectroscopy

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UV-Vis Spectroscopy. The solutions of mTHPC, apoMb, and mTHPC@apoMb were characterized through UV-Vis spectroscopy. The absorption spectra were collected using a Cary60 UV-Vis spectrophotometer (Agilent Technologies, Stockport, UK).
Fluorescence Spectroscopy. The fluorescence spectra of mTHPC and mTHPC@apoMb were acquired with an Edinburgh FLS920 equipped with a photomultiplier Hamamatsu R928P.
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2

Synthesis and Characterization of Nanoparticles

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Unless mentioned, all chemicals were purchased from Sigma Aldrich (Merck) and used without further purification. Wherever required, the solvents were dried following general procedures. The FT-IR spectra of the samples were obtained using a Bruker Tensor II spectrometer by pasting the sample in a NaCl window. The identification of phase and crystal structures was performed using an analytical powder X-ray diffractometer (XRD) in the range of 20–70° (2θ) with Ni-filtered Cu Kα (1.5405 Å) radiation at 40 kV and 30 mA. The sampling was done by pasting the sample in a glass slide using methanol as a diluter and drying. The luminescence spectrum studies for nanoparticle samples were carried out using the Edinburgh instrument FLS920 attached to a Hamamatsu R955 photomultiplier tube. Other luminescence measurements (in tubes, cells and animals) were carried out using the Live Animal Imaging System (Photon Imager, Biospace, France).
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3

Photothermal Effect Characterization Protocol

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The PT effect was evaluated by the setup shown in Figure S1, where an 808 nm laser diode (LD) and a ZnSe infrared thermometer (LumaSense IMPAC, Germany), respectively, were used to illuminate samples and monitor the temperature of the sample surface in air in a range from 273 to 1173 K. Here, the 808 nm laser was selected as the irradiation source to avoid water absorption in tissues and improve the tissue-penetration depth33 (link),34 (link). The temperature of the samples immersed in simulated body fluid (SBF) solution was measured with a digital thermometer (Kangyou, Nanjing, China). Optical absorption spectra were measured with a Perkin Elmer Lambda 900 UV/Vis/NIR spectrophotometer in a spectral range from 200 to 3200 nm. PL spectra were recorded with a Zolix Omni λ3007 spectrometer equipped with an InGaAs photodetector and a SR830 Stanford lock-in amplifier. The fluorescence lifetimes were measured with a digital phosphor oscilloscope (TDS3012C, Tektronix, America). The excitation spectra were measured with an Edinburgh Instrument FLS 920 (Livingston, WL, UK) equipped with a liquid nitrogen-cooled photomultiplier (Hamamatsu R5509-72, Hamamatsu, Japan).
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