Spr 320

Manufactured by Millar

Sourced in United States

The SPR-320 is a surface plasmon resonance (SPR) instrument designed for real-time monitoring of biomolecular interactions. It is a highly sensitive analytical tool used for the detection and characterization of various molecular binding events.

Automatically generated - may contain errors

Lab products found in correlation

Powerlab 8 30, by ADInstruments (5 mentions) Vevo 770 echocardiography machine, by Fujifilm (5 mentions) Pvan3, by Millar (5 mentions) Mpvs 300, by Millar (5 mentions) Power lab data interface module, by ADInstruments (2 mentions) Model 683, by Harvard Apparatus (1 mentions) Acqknowledge 3, by Biopac (1 mentions) Hp sonos 5500, by Hewlett-Packard (1 mentions) Coda system, by Kent Scientific (1 mentions) Powerlab system, by ADInstruments (1 mentions) Powerlab 4sp, by ADInstruments (1 mentions) Bridge amp, by ADInstruments (1 mentions) Tc 510, by Millar (1 mentions) Dx 200, by Nihon Kohden (1 mentions) Ma 2.5psb, by Transonic Systems (1 mentions) Urethane, by Merck Group (1 mentions) Powerlab 4 30, by ADInstruments (1 mentions) Bp 98a, by Softron (1 mentions) Labchart 7, by ADInstruments (1 mentions)

18 protocols using spr 320

Echocardiography and Hemodynamics in Myocardial Infarction

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transthoracic echocardiography was performed assessed pre-myocardial infarction (base-line) and at day 28 post-treatment by the Vevo-770 echocardiography machine (VisualSonics, Fujifilm, Amsterdam, Netherlands) under isoflurane inhalation administered via a nose cone as we have previously described [[14] , [15] (link), [16] (link),31 (link)]. LV end-diastolic (LVEDD) and end-systolic (LVESD) dimensions, under stable, consistent heart rate, were measured using M mode. Left ventricular ejection fraction (LVEF) was calculated from the data obtained with 2-dimensional tracing. All data were collected blind from at least 3–5 different measurements.
Hemodynamic parameters were measured by using cardiac catheterization (SPR-320 and PVAN3.2; Millar Instruments, USA) as we have previously described [[14] , [15] (link), [16] (link),31 (link)]. Briefly, under general anesthesia using isoflurane inhalation with a nose cone, the catheter was inserted into the left ventricular cavity through the right common carotid artery. Intra-LV pressure signals were measured with a transducer and conditioner (MPVS-300; Millar Instruments) and digitally recorded with a data acquisition system (PowerLab 8/30; AD Instruments, Oxford, UK). All data were collected from at least 5 different measurements in a blinded manner.

Kobayashi K., Ichihara Y., Sato N., Umeda N., Fields L., Fukumitsu M., Tago Y., Ito T., Kainuma S., Podaru M., Lewis-McDougall F., Yamahara K., Uppal R, & Suzuki K. (2019). On-site fabrication of Bi-layered adhesive mesenchymal stromal cell-dressings for the treatment of heart failure. Biomaterials, 209, 41-53.

+ Open protocol

+ Expand

Echocardiography and Cardiac Catheterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transthoracic echocardiography was performed at 4 weeks after left coronary artery ligation (for pre-treatment data) and at day 28 post-treatment using Vevo-770 echocardiography machine (VisualSonics, Amsterdam, Netherlands) under 1.5% isoflurane inhalation via a nose cone^{3 (link),4}. LVEF and LVFS were calculated from the data obtained with 2-dimensional tracing. All data were collected from at least 3–5 different measurements in a blinded manner. In addition, hemodynamic parameters were measured by using cardiac catheterization (SPR-320 and PVAN3.2; Millar Instruments, Houston, TX) as previously described^{3 (link),4}. Briefly, under general anesthesia using 1.5% isoflurane inhalation and mechanical ventilation, the catheter was inserted into the left ventricular cavity through the right common carotid artery. Intra-LV and intra-aortic pressure signals were measured with a transducer and conditioner (MPVS-300; Millar Instruments) and digitally recorded with a data acquisition system (PowerLab 8/30; ADInstruments, Oxford, UK). All data were collected from at least 5 different measurements per animal in a blinded manner.

Kobayashi K., Ichihara Y., Tano N., Fields L., Murugesu N., Ito T., Ikebe C., Lewis F., Yashiro K., Shintani Y., Uppal R, & Suzuki K. (2018). Fibrin Glue-aided, Instant Epicardial Placement Enhances the Efficacy of Mesenchymal Stromal Cell-Based Therapy for Heart Failure. Scientific Reports, 8, 9448.

+ Open protocol

+ Expand

Invasive Hemodynamic Measurements in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 8, 12 and 16 weeks after dietary intervention, the rats were anesthetized (sodium pentobarbital, 60 mg/kg, intraperitoneally) for invasive hemodynamics using a 2F high fidelity micro-tip pressure catheter (SPR-320; Millar Instruments Ltd.) to measure systolic and diastolic BP (SBP and DBP), LV end-diastolic pressure (LVEDP), maximal slope of systolic pressure increment (+dP/dt_max), and diastolic pressure decrement (-dP/dt_min) as previously described by the authors (12 (link)).

Bi Y., Yang G.H., Guo Z.Z., Cai W., Chen S.B., Zhou X, & Li Y.M. (2023). Chronic high‑salt intake induces cardiomyocyte autophagic vacuolization during left ventricular maladaptive remodeling in spontaneously hypertensive rats. Experimental and Therapeutic Medicine, 25(4), 148.

+ Open protocol

+ Expand

Echocardiographic Assessment of Rat Infarcts

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

As previously described^{5 (link)}, transthoracic echocardiographic was performed using an HP Sonos-5500 (Hewlett Packard, Andover, MA, USA) echocardiography. Rats were imaged following 2- and 30-days post-infarction, in which only rats with large infarcts ( ≥ 37% of LV) were included. Immediately after the last echocardiography, the rats were intubated, ventilated (rodent ventilator, model 683, Harvard Apparatus, Holliston, MA, USA) and a 2-F gauge Millar catheter-tip micromanometer SPR-320 (Millar Instruments, Houston, TX, USA) was inserted through the right carotid artery into the LV cavity^{4 (link)}. Measurements of LV parameters, including heart rate (HR), LV systolic pressure (LVSP), LV end-diastolic pressure (LVEDP), and maximal positive ( + dP/dt) and negative (-dP/dt) time derivatives of the developed pressure were studied using AcqKnowledge 3.5.7 software (Biopac Systems Inc., Santa Barbara, CA, USA).

Grandinetti V., Carlos F.P., Antonio E.L., de Oliveira H.A., dos Santos L.F., Yoshizaki A., Mansano B.S., Silva F.A., Porte L.A., Albuquerque-Pontes G.M., de Carvalho P.D., Manchini M.T., Leal-Junior E.C., Tucci P.J, & Serra A.J. (2019). Photobiomodulation therapy combined with carvedilol attenuates post-infarction heart failure by suppressing excessive inflammation and oxidative stress in rats. Scientific Reports, 9, 9425.

+ Open protocol

+ Expand

Invasive Cardiac Hemodynamics Monitoring

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Invasive real time recording of cardiac hemodynamics was carried out according to the method described in a previous report from our laboratories [13 (link)]. Following anaesthesia as described above the animals’ body temperature was maintained at 37°C (measured by a rectal probe) using controlled heating pads. A microtip pressure transducer (SPR-320, Millar Instruments, Houston, TX, USA) was inserted through a small incision into the right carotid artery and advanced into the left ventricle. After 5 min stabilization period, the signals were continuously recorded. The microtip catheter was connected to a Power Lab Data Interface Module connected to a PC running Lab Chart professional software (v8.0, AD Instruments, Bella Vista, Australia) including the BP module. The BP module detects and calculates the left ventricular end ventricular systolic pressure (ESP), left ventricular end-diastolic pressure (EDP), slope of the systolic pressure increment (dP/dt) and slope of the diastolic pressure decrement (−dP/dt), cystolic and diastolic duration, and contractility index.

El-Bassossy H.M, & Watson M.L. (2015). Xanthine oxidase inhibition alleviates the cardiac complications of insulin resistance: effect on low grade inflammation and the angiotensin system. Journal of Translational Medicine, 13, 82.

+ Open protocol

+ Expand

Measuring Aortic Blood Pressure

Check if the same lab product or an alternative is used in the 5 most similar protocols

Systemic blood pressure was measured in the conscious status by restraint tail cuff every 2 days for 2–3 weeks using the CODA system (Kent Scientific, CT). Direct aortic pressure was measured in ascending thoracic aorta via inserting a Millar catheter (SPR 320, Millar Instruments, TX) through right common carotid artery under anaesthesia with an inspired concentration of 2% isoflurane (JD Medical, AZ). The transducer was connected to a Powerlab system (AD Instruments, Castle Hill, Australia) to record systolic aortic pressure (SAP) and diastolic aortic pressure (DAP). Mean aortic pressure (MAP) and pulse pressure (PP) were then calculated accordingly (MAP = DAP + (SAP−DAP)/3, PP= SAP−DAP).

Zhou N., Lee J.J., Stoll S., Ma B., Wiener R., Wang C., Costa K.D, & Qiu H. (2016). Inhibition of SRF/myocardin reduces aortic stiffness by targeting vascular smooth muscle cell stiffening in hypertension. Cardiovascular Research, 113(2), 171-182.

+ Open protocol

+ Expand

Assessing Left Ventricular Function in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

Four weeks after MI induction, rats were anaesthetized initially with a 5% isoflurane/O₂ mixture in an induction box and then kept asleep with a 2.5% isoflurane/O₂ mixture via a face mask attached to a gas vaporizer during spontaneous ventilation. Rats were placed in the supine position, and body temperature was maintained at 37°C by a heating pad throughout the experiment. Left ventricular function was measured using a pressure catheter (SPR-320; Millar Instruments, Houston, TX, USA), which was inserted into the right carotid artery. After 10 min stabilization, arterial blood pressure was recorded for 10 min. Then, the catheter was advanced into the left ventricle and stabilized for 5 min. The waveform was used to confirm the positioning of catheter in the LV. Left ventricular end-diastolic pressure (LVEDP) was continuously recorded for 10 min by the Powerlab Chart 4 software system (ADInstruments Inc., Colorado Springs, CO, USA).

Li Calzi S., Cook T., Della Rocca D.G., Zhang J., Shenoy V., Yan Y., Espejo A., Rathinasabapathy A., Jacobsen M.H., Salazar T., Sandusky G.E., Shaw L.C., March K., Raizada M.K., Pepine C.J., Katovich M.J, & Grant M.B. (2019). Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model. Stem Cells International, 2019, 3945850.

+ Open protocol

+ Expand

Ventricular Pressure Measurement and Dobutamine Challenge

Check if the same lab product or an alternative is used in the 5 most similar protocols

Under the anesthesia of urethane (1 g/kg, i.p.), a catheter specially designed to measure ventricular pressure (SPR 320: Millar Instruments, Houston, TX, USA) was inserted into the LV via the right carotid. The catheter was connected, through a dedicated signal coupler (TC-510, Millar Instruments, USA), to a recording system (Bridge Amp attached to PowerLab/4SP, AD Instruments, Sydney, Australia) and, under continuous recording of LV pressure, they received increasing doses of dobutamine (1, 3, 10 and 15 μg/kg) intravenously. Dobutamine was used as a pharmacological stressor, and each dose was injected, in bolus, with an interval of at least 10 min between each other. The 1^st derivative in time of the LV pressure (dP/dt) was calculated online, and the maximum rate of increasing pressure was used as an index of systolic function of the rats.

Omoto A.C., Gava F.N., Silva C.A., Silva H.B., Parente J.M., Costa R.M., Castro M.M., Tostes R.D., Salgado H.C, & Fazan R J.r. (2018). Lack of scarring is not always a sign of cardiac health: Functional and molecular characterization of the rat heart’s following chronic reperfusion. PLoS ONE, 13(12), e0209190.

+ Open protocol

+ Expand

Invasive Cardiac Contractility Monitoring

Check if the same lab product or an alternative is used in the 5 most similar protocols

Invasive real-time recording of cardiac contractility was carried out according to the method described by Radovits et al [14] (link) with slight modifications. After urethane anaethesia, animals were placed on controlled heating pads, and body temperature, measured via a rectal probe, was maintained at 37°C. A microtip pressure transducer (SPR-320; Millar Instruments, Houston, TX, USA) was inserted into the right carotid artery and advanced into the left ventricle under pressure control. After stabilization for 5 min, the signals were continuously recorded at a sampling rate of 1000 s⁻¹. The microtip catheter was connected to a PowerLab Data Interface Module connected to a PC running LabChart professional software (version 7.3; ADI Instruments, Bella Vista, Australia) containing a blood pressure module, which detects and calculates the slopes of the systolic pressure increment (dP/dt) and the diastolic pressure decrement (−dP/dt), the systolic and diastolic duration, and the contractility index.

Azhar A, & El-Bassossy H.M. (2014). Pentoxifylline Alleviates Cardiac Ischemia and Dysfunction Following Experimental Angina in Insulin Resistance. PLoS ONE, 9(5), e98281.

+ Open protocol

+ Expand

Echocardiographic and Hemodynamic Evaluation after MI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transthoracic echocardiographic determinations were assessed before treatment (4 weeks after MI) and at 28 days after treatment by the Vevo 770 echocardiography machine (VisualSonics) under 1.5% isoflurane inhalation via a nose cone.11, 13, 14 LV ejection fraction was calculated from the data obtained with 2‐dimensional tracing. LV end‐diastolic and end‐systolic dimensions, LV wall thickness, and heart rate were measured with M mode. Transmitral peak E/A flow ratio was determined by spectral Doppler traces. All data were collected from at least 3 to 5 different measurements in a blinded manner.
Hemodynamic parameters were measured by using cardiac catheterization (SPR‐320 and PVAN3.2; Millar Instruments), as described previously.11, 13, 14 Briefly, under general anesthesia using 1.5% isoflurane inhalation and mechanical ventilation, the catheter was inserted into the LV cavity through the right common carotid artery. Intra‐LV and intra‐aortic pressure signals were measured with a transducer and conditioner (MPVS‐300; Millar Instruments) and digitally recorded with a data acquisition system (PowerLab 8/30; ADInstruments). All data were collected from at least 5 different measurements in a blinded manner.

Tano N., Kaneko M., Ichihara Y., Ikebe C., Coppen S.R., Shiraishi M., Shintani Y., Yashiro K., Warrens A, & Suzuki K. (2016). Allogeneic Mesenchymal Stromal Cells Transplanted Onto the Heart Surface Achieve Therapeutic Myocardial Repair Despite Immunologic Responses in Rats. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(2), e002815.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!