Cryo-TEM imaging was done on a FEI G2 F30 Tecnai TEM operated at 200 kV to obtain high-resolution imaging of liposomes prepared by extrusion 21 times through a 100 nm polycarbonate membrane and liposomes within the cluster. The sample was prepared by using a FEI Vitrobot. Five microliters of the sample was applied to a 200-mesh lacey carbon grid (Electron Microscopy Sciences). Excess liquid was blotted by filter paper attached to the arms of the Vitrobot for 2 s to form a thin film. The sample was then vitrified by plunging into liquid ethane followed by liquid nitrogen. The vitrified sample was finally transferred onto a single-tilt cryo-specimen holder for imaging. The cryo-holder was maintained below 170 ° C to prevent sublimation of vitreous water.
200 mesh lacey carbon grid
Manufactured by Electron Microscopy Sciences
The 200-mesh lacey carbon grid is a type of sample preparation substrate used in electron microscopy. It features a 200-mesh copper grid with a lacey carbon support film. The lacey carbon film provides a porous and delicate structure that can be used for the analysis of small or fragile specimens.
Automatically generated - may contain errors
3 protocols using 200 mesh lacey carbon grid
1
Cryo-TEM Imaging of Extruded Liposomes
2
Cryo-TEM Imaging of Nanoparticle Complexes
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The morphology
of the complexes was characterized
by a FEI Tecnai G2 F30 twin transmission electron microscope operated
at 300 kV equipped with SDD EDS for elemental mapping. Cryo-TEM imaging
was done on an FEI G2 F30 Tecnai TEM operated at 150 kV. To prepare
the sample, a 200-mesh lacey carbon grid (Electron Microscopy Sciences)
was picked up with tweezers and mounted on the plunging station of
an FEI Vitrobot. Four microliters of the solution was applied to the
grid. The excess liquid was blotted by filter paper attached to arms
of the Vitrobot for 2 s to form a thin film. The sample was then vitrified
by plunging into liquid ethane. The vitrified sample was finally transferred
onto a single-tilt cryo specimen holder for imaging.
of the complexes was characterized
by a FEI Tecnai G2 F30 twin transmission electron microscope operated
at 300 kV equipped with SDD EDS for elemental mapping. Cryo-TEM imaging
was done on an FEI G2 F30 Tecnai TEM operated at 150 kV. To prepare
the sample, a 200-mesh lacey carbon grid (Electron Microscopy Sciences)
was picked up with tweezers and mounted on the plunging station of
an FEI Vitrobot. Four microliters of the solution was applied to the
grid. The excess liquid was blotted by filter paper attached to arms
of the Vitrobot for 2 s to form a thin film. The sample was then vitrified
by plunging into liquid ethane. The vitrified sample was finally transferred
onto a single-tilt cryo specimen holder for imaging.
3
Cryo-TEM Imaging of Vitrified Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cryo-TEM imaging
was done on a FEI G2 F30 Tecnai TEM operated at 200 kV. The sample
was prepared using a FEI Vitrobot. Five microliters of the solution
were applied to a 200-mesh lacey carbon grid (Electron Microscopy
Sciences). Excess liquid was blotted by filter paper attached to the
arms of the Vitrobot for 2 s to form a thin film. The sample was then
vitrified by plunging into liquid ethane followed by liquid nitrogen.
The vitrified sample was finally transferred onto a single tilt cryo-specimen
holder for imaging. The cryo-holder was maintained below 170 °C
to prevent sublimation of vitreous water. All size analyses on cryo-TEM
images were carried out using ImageJ software.
was done on a FEI G2 F30 Tecnai TEM operated at 200 kV. The sample
was prepared using a FEI Vitrobot. Five microliters of the solution
were applied to a 200-mesh lacey carbon grid (Electron Microscopy
Sciences). Excess liquid was blotted by filter paper attached to the
arms of the Vitrobot for 2 s to form a thin film. The sample was then
vitrified by plunging into liquid ethane followed by liquid nitrogen.
The vitrified sample was finally transferred onto a single tilt cryo-specimen
holder for imaging. The cryo-holder was maintained below 170 °C
to prevent sublimation of vitreous water. All size analyses on cryo-TEM
images were carried out using ImageJ software.
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