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Cd93 pe

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CD93-PE is a fluorescently-labeled antibody that binds to the CD93 cell surface receptor. It is a tool for the identification and analysis of CD93-expressing cells in flow cytometry applications.

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Lab products found in correlation

Facsaria, by BD (2 mentions) Facscanto, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Annexin 5 pacific blue, by Thermo Fisher Scientific (1 mentions) Cd3 fitc, by Thermo Fisher Scientific (1 mentions) Cd3 biotin, by Thermo Fisher Scientific (1 mentions) Ter119 biotin, by Thermo Fisher Scientific (1 mentions) Facsdiva, by BD (1 mentions) Canto 2 cytometer, by BD (1 mentions) 7 aad, by Thermo Fisher Scientific (1 mentions) Cd23 pe cy7, by Thermo Fisher Scientific (1 mentions) Cd21 fitc, by Thermo Fisher Scientific (1 mentions) B220 eflour605, by Thermo Fisher Scientific (1 mentions) Gr1 biotin, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD93-PE-Cy7 (2 citations) PE-anti-IgM (eB121-15F9); APC anti-CD93 (AA4.1); FITC-anti-F4/80 (BM8) (2 citations)

3 protocols using cd93 pe

1

Immunophenotyping of Normal and CML Cells

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Cells were stained using the following antibody cocktail (all BD Biosciences apart from CD93-PE from eBioscience); lineage cocktail-FITC [CD3 (MφP9), CD14 (3G8), CD16 (NCAM16.2), CD19 (SJ25C1), CD20 (SK7), CD56 (L27)], CD34-PerCP (8G12), CD38-V450 (HIT2), CD45RA-APC H7 (HI100), CD90-PE Cy7 (5E10), CD123-APC (7G3), and CD93-PE (R3). Immunophenotypic analysis and cell sorting of normal and CML samples was performed following antibody staining on a FACSCanto or FACSAria (BD Biosciences). FACS data were analyzed with FACS Diva software (Becton Dickinson) or FlowJo (TreeStar).
Kinstrie R., Horne G.A., Morrison H., Irvine D., Munje C., Castañeda E.G., Moka H.A., Dunn K., Cassels J.E., Parry N., Clarke C.J., Scott M.T., Clark R.E., Holyoake T.L., Wheadon H, & Copland M. (2020). CD93 is expressed on chronic myeloid leukemia stem cells and identifies a quiescent population which persists after tyrosine kinase inhibitor therapy. Leukemia, 34(6), 1613-1625.
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2

Immunophenotypic analysis of normal and CML cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were stained using the following antibody cocktail (all BD Biosciences apart from CD93-PE from eBioscience); lineage cocktail-FITC [CD3 (MϕP9), CD14 (3G8), CD16 (NCAM16.2), CD19 (SJ25C1), CD20 (SK7), CD56 (L27)], CD34-PerCP (8G12), CD38-V450 (HIT2), CD45RA-APC H7 (HI100), CD90-PE Cy7 (5E10), CD123-APC (7G3) and CD93-PE (R3). Immunophenotypic analysis and cell-sorting of normal and CML samples was performed following antibody staining on a FACSCanto or FACSAria (BD Biosciences). FACS data were analyzed with FACS Diva software (Becton Dickinson) or FlowJo (TreeStar).
Kinstrie R., Horne G.A., Morrison H., Irvine D., Munje C., Castañeda E.G., Moka H.A., Dunn K., Cassels J.E., Parry N., Clarke C.J., Scott M.T., Clark R.E., Holyoake T.L., Wheadon H, & Copland M. (2020). CD93 is expressed on chronic myeloid leukemia stem cells and identifies a quiescent population which persists after tyrosine kinase inhibitor therapy. Leukemia, 34(6), 1613-1625.
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3

Multiparametric Flow Cytometric Analysis

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Cell suspensions of different organs were lysed with ammonium chloride buffer (0.150 mM NH4Cl, 0.1 mM EDTA, 0.150 mM KHCO3) to eliminate erythrocytes followed by staining with CD19-FITC/APC (eBio1D3, 1:100), CD38-PE (clone 90, 1:100), IgM-PE/PECy7 (II/41, 1:200), IgD-eFlour450 (11-26c, 1:30), CD5-APC/Pacific Blue (53-7.3, 1:80), B220-eFlour605 (RA3-6B2, 1:40), CD3-FITC (145-2C11, 1:100), ZAP70-FITC (1E7.2, 1:20), CD21-FITC (eBio8D9, 1:100), CD23-PECy7 (B3B4, 1:160), CD93-PE (AA4.1, 1:80), LIN-Cocktail (CD11b-Biotin (M1/70, 1:130), CD3-Biotin (145-2C11, 1:130), Ter119-Biotin (Ter119, 1:130) and Gr1-Biotin (RB6-8C5, 1:130), Streptavidin-PerCP (1:160), Annexin V-Pacific Blue (1:25) and 7-AAD (1:100) (all from eBioscience). Cells were analysed with the Canto II cytometer (BD Bioscience). Data was obtained with the BD FACSDivaTM (BD) and analysed with the FlowJo 8.5.3 software. The sequential gating strategy for all FACS panels is provided in Supplementary Fig. 11.
Märklin M., Heitmann J.S., Fuchs A.R., Truckenmüller F.M., Gutknecht M., Bugl S., Saur S.J., Lazarus J., Kohlhofer U., Quintanilla-Martinez L., Rammensee H.G., Salih H.R., Kopp H.G., Haap M., Kirschniak A., Kanz L., Rao A., Wirths S, & Müller M.R. (2017). NFAT2 is a critical regulator of the anergic phenotype in chronic lymphocytic leukaemia. Nature Communications, 8, 755.
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