The following antibodies were used: Pol II 4H8 specific to phospho S5 of the C-terminal domain repeat region (AbCam #ab5408), TBP (AbCam #ab51841), Sp1 (SantaCruz #sc-17824), Med23 (BD Pharmingen #550429), Med1 (Bethyl #A300-793A), H3K4me3 (Abcam #ab12209), H3K27me3 (AbCam #ab6002), H3K27acetyl (AbCam #ab4729), H3K9me3 (AbCam #ab176916), H3 (AbCam #1791), and ICP4 58S (derived from hybridomas-ATCC HB8183).
Ab51841
Manufactured by Santa Cruz Biotechnology
Ab51841 is a protein detection reagent manufactured by Santa Cruz Biotechnology. The product is designed for use in standard immunochemical techniques.
Automatically generated - may contain errors
2 protocols using ab51841
1
Chromatin Immunoprecipitation Assay
2
ChIP-Seq Protocol for Transcription Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ChIP-Seq was performed as described previously (32 (link)). Samples were immunoprecipitated with 25 µg of the following antibodies: Pol II 4H8 (AbCam #ab5408), Pol II 8WG16 (AbCam #ab817), TBP (AbCam #ab51841), TAF1 (SantaCruz #sc-735), or Sp1 (SantaCruz #sc-17824). Libraries were generated from 2 to 20 ng of using NEBNext kit, #E7103S.
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