Calu 3 cell line

Vero E6 cell line (Cat # CRL-1586, gender unknown) cell line was purchased from American Type Culture Collection (ATCC) and cultured at 37°C with 5% CO₂in eagle’s minimal essential medium (MEM) supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin and L-glutamine. Calu-3 cell line (Cat # HTB-55, obtained from a male, lung adenocarcinoma patient) was obtained from ATCC and maintained in EMEM+20%FBS at 37°C with 5% CO₂.
The SARS-CoV-2 isolate hCoV-19/USA/MD-HP20874/2021 was obtained from BEI Resources, NIAID, NIH, and had been tittered on Vero E6 cells by plaque assay.

The Vero E6 cell line (ATCC CRL-1586) and the Caco-2 cell line (ATCC HTB-37) were purchased from the American Type Culture Collection (ATCC) and cultured in Eagle’s minimal essential medium (EMEM) supplemented with 10% fetal bovine serum (FBS; Invitrogen), 1% penicillin-streptomycin, and l-glutamine. The Calu-3 cell line (ATCC HTB-55) was obtained from the ATCC and maintained in EMEM plus 20% FBS. The Huh7.5.1 cell line was a gift of F. Chisari and was maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus 10% FBS (41 (link)).
The SARS-CoV-2 isolate USA-WA1/2020 was obtained from BEI Resources, NIAID, NIH, and had been passed three times on Vero cells and one time on Vero E6 cells prior to acquisition. It was further passaged once on Vero E6 cells in our laboratory. The virus has been sequence verified to contain no mutation in its original seed virus. A reporter virus (icSARS-CoV-2-mNG) that contains an insert of monomeric neon green (mNG) in the SARS-CoV-2 USA-WA1/2020 backbone was obtained from Pei-Yong Shi’s laboratory (University of Texas Medical Branch) at passage 2 and was further expanded once on Vero E6 cells (28 (link)) at the FDA.