CH-223191 was purchased from Selleck. L-kynurenine and BAY11-7085 were purchased from MedChemExpress. Antibodies were used as following: AHR (D5S6H; Cell Signaling Technology), RelA (D14E12; Cell Signaling Technology), IκBα (L35A5; Cell Signaling Technology), phospho-IκBα (14D4; Cell Signaling Technology), α-Tubulin (DM1A; Cell Signaling Technology), LaminB1 (D9V6H; Cell Signaling Technology), HTLV-1 Tax (1A3; Abcam), HTLV-1 gp46 (67/5.5.13.1; Abcam), HTLV-1 p24 (46/3.24.4; Abcam), HTLV-1 p19 (TP-7; Abcam), β-Actin (AF0003; Beyotime Biotechnology), GAPDH (AF0006; Beyotime Biotechnology).
Rela d14e12
Manufactured by Cell Signaling Technology
RelA (D14E12) is a rabbit monoclonal antibody that recognizes the RelA (p65) subunit of the NF-kB transcription factor complex. RelA is a core component of the NF-kB signaling pathway, which plays a central role in regulating cellular responses to various stimuli, including inflammation, immunity, cell growth, and survival.
Automatically generated - may contain errors
Lab products found in correlation
Iκbα l35a5, by Cell Signaling Technology (2 mentions)
Ahr d5s6h, by Cell Signaling Technology (1 mentions)
Ch 223191, by Selleck Chemicals (1 mentions)
Bay 11 7085, by MedChemExpress (1 mentions)
Phospho iκbα 14d4, by Cell Signaling Technology (1 mentions)
α tubulin dm1a, by Cell Signaling Technology (1 mentions)
Laminb1 d9v6h, by Cell Signaling Technology (1 mentions)
β actin, by Beyotime (1 mentions)
Gapdh, by Beyotime (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Rockland Immunochemicals (1 mentions)
Actin d6a8, by Cell Signaling Technology (1 mentions)
Criterion tgx precast gel, by Bio-Rad (1 mentions)
Myc 9e10, by Santa Cruz Biotechnology (1 mentions)
Gfp d5.1, by Cell Signaling Technology (1 mentions)
Vinculin h10, by Santa Cruz Biotechnology (1 mentions)
G box chemi detection system, by Syngene (1 mentions)
Sds sample buffer, by Boston BioProducts (1 mentions)
Ripa buffer, by Boston BioProducts (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
Clarity western ecl substrate, by Bio-Rad (1 mentions)
2 protocols using rela d14e12
1
Investigating HTLV-1 Activation Pathway
2
Western Blot Analysis of Cellular Protein Markers
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The following antibodies were used: MCPyV Ab5 [36 (link), 57 (link)]; GFP (D5.1, Cell Signaling); vinculin (H-10, Santa Cruz); actin (D6A8, Cell Signaling); HK2 (C64G5, Cell Signaling); LDHA (EP1565Y, Abcam); MCT1 (A1512, NeoBiolab); LAT1 (5347, Cell Signaling); RelA (D14E12, Cell Signaling); IκBα (L35A5, Cell Signaling); MYC (9E10, Santa Cruz); MYCN (9405, Cell Signaling); MYCL (AF4050, R&D Systems).
Whole cell lysates were prepared using RIPA buffer (Boston BioProducts) supplemented with protease inhibitor cocktail set I (Calbiochem) and phosphatase inhibitor cocktail set I (Calbiochem). Clarified protein extracts were boiled in SDS sample buffer (Boston BioProducts), resolved by SDS-PAGE (Criterion TGX precast gels; Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad), blocked and incubated with the appropriate primary antibody in TBS-T overnight at 4°C. Detection of proteins was performed with horseradish peroxidase-conjugated secondary antibodies (Rockland), developed using Clarity Western ECL substrate (Bio-Rad), and imaged with a G:BOX Chemi detection system (Syngene).
Whole cell lysates were prepared using RIPA buffer (Boston BioProducts) supplemented with protease inhibitor cocktail set I (Calbiochem) and phosphatase inhibitor cocktail set I (Calbiochem). Clarified protein extracts were boiled in SDS sample buffer (Boston BioProducts), resolved by SDS-PAGE (Criterion TGX precast gels; Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad), blocked and incubated with the appropriate primary antibody in TBS-T overnight at 4°C. Detection of proteins was performed with horseradish peroxidase-conjugated secondary antibodies (Rockland), developed using Clarity Western ECL substrate (Bio-Rad), and imaged with a G:BOX Chemi detection system (Syngene).
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