Donepezil hydrochloride monohydrate (≥98%), berberine chloride dihydrate (≥98%), AChE from electric eel (EC 3.1.1.7, 518 U mg−1), ATCh, poly(lactic acid), thionine acetate (3,7-diamino-5-phenothiazinium acetate), Methylene blue (MB, 3,7-bis(dimethylamino)phenazathionium chloride), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide chloride (EDC), N-hydroxysuccinimide (NHS), pralidoxime (2-pyridine aldoxime methiodide, 2-PAM), and carbofuran (2,3-dihydro-2,2-dimethylbenzofuran-7-yl methylcarbamate) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Unsubstituted P[5]A was synthesized at the Organic Chemistry Department of Kazan Federal University by the modified Ogoshi method [42 (link)]. Carbon black N 220 (CB, >99.95% C) was purchased from Imerys Graphite&Carbon (Willebroek, Belgium). All the working solutions were prepared using Millipore Q® water (Simplicity® water purification system, Merck-Millipore, Molsheim, France). Other reagents were of analytical grade.
Artificial urine contained 10 mM CaCl2, 6 mM MgCl2, 6 mM Na2SO4, 2 mM potassium citrate, 20 mM KH2PO4, 21 mM KCl, 18 mM NH4Cl, 9 mM creatinine and 416 mM urea.
Electrochemical measurements were performed in a 0.1 M phosphate buffer containing 0.1 M KCl.
Artificial urine contained 10 mM CaCl2, 6 mM MgCl2, 6 mM Na2SO4, 2 mM potassium citrate, 20 mM KH2PO4, 21 mM KCl, 18 mM NH4Cl, 9 mM creatinine and 416 mM urea.
Electrochemical measurements were performed in a 0.1 M phosphate buffer containing 0.1 M KCl.