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Fsp1 cre

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FSP1-Cre is a Cre recombinase-expressing transgene. Cre recombinase is an enzyme that recognizes and binds to specific DNA sequences called loxP sites, and catalyzes the excision or inversion of the DNA between the loxP sites. The FSP1-Cre transgene expresses Cre recombinase under the control of the fibroblast-specific protein 1 (FSP1) promoter, which drives expression in fibroblasts and some other cell types.

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Lab products found in correlation

Actb tdtomato egfp, by Jackson ImmunoResearch (2 mentions) Mmtv pymt, by Jackson ImmunoResearch (2 mentions) Mmtv neu, by Jackson ImmunoResearch (2 mentions) Cb17 scid mice, by Charles River Laboratories (2 mentions) Rosa26creer, by Jackson ImmunoResearch (2 mentions) β actin egfp mice, by Jackson ImmunoResearch (2 mentions) Tamoxifen tam, by Merck Group (2 mentions) Bleomycin, by Merck Group (1 mentions) R26rtdtomato, by Jackson ImmunoResearch (1 mentions) R26rtdtomato mice, by Jackson ImmunoResearch (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Fsp1 cre mice, by Jackson ImmunoResearch (1 mentions) Vav1 cre, by Jackson ImmunoResearch (1 mentions) R26 smo m2, by Jackson ImmunoResearch (1 mentions) Mt mg, by Jackson ImmunoResearch (1 mentions)

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Fsp1-Cre mice (8 citations) Fsp1-Cre transgenic mice (2 citations)

9 protocols using fsp1 cre

1

Fibroblast-specific Rcn3 deletion in lung fibrosis

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All animal procedures were approved by the Animal Care and Ethics Committee of the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and were performed in accordance with the Guide for the Care and Use of Laboratory Animals of the Chinese Academy of Sciences. By crossing the fibroblast-specific protein (FSP)1-Cre (Jackson Lab) and Rcn3flox/flox mice (generated in our lab [21 (link)]), the fibroblast-selective Rcn3 deletion mice (CKO, FSP1-Cre/Rcn3f/f) were generated and the littermates (Rcn3flox/flox) served as controls. The intratracheal administration of bleomycin (Sigma-Aldrich) was performed in 8-week-old mice at the dose of 0.08 U/kg in 50 μl saline (25 μl × 2), and sterile saline was instilled at the same procedure as a control [21 (link)].
Wu M., Wang Z., Shi X., Zan D., Chen H., Yang S., Ding F., Yang L., Tan P., Ma R.Z., Wang J., Ma L., Ma Y, & Jin J. (2023). TGFβ1-RCN3-TGFBR1 loop facilitates pulmonary fibrosis by orchestrating fibroblast activation. Respiratory Research, 24, 222.
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2

Isolating Fibroblast Lineage Cells

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MEFs were isolated from E12.5. Gonads and internal organ were removed before processing for isolation of MEF cells. MEFs were growth in fibroblast growth medium (FGM) consisting of DMEM (Gibco), 15% fetal bovine serum (FBS, Gibco), 2 mM GlutaMAX (Gibco), 0.1 mM non-essential amino acids (NEAA) (Gibco), 100 units/mL penicillin, and 100 μg/mL streptomycin. For lineage-tracing experiments the Fsp1-Cre mice (Jackson Laboratory) were mated with R26RtdTomato mice (Jackson Laboratory) and isolated the Fsp1-Cre:R26RtdTomato (td-tomato-positive) MEFs from E12.5. TdTomato+-TTFs were isolated from adult (8–10 weeks) Fsp1-Cre:R26RtdTomato mice. Tail tips were cut into pieces and then dispersed on gelatin-coated 10 cm culture dishes containing 2 mL FGM, and an additional 8 mL FGM was supplemented on the next day.
Guo R., Tang W., Yuan Q., Hui L., Wang X, & Xie X. (2017). Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor. Stem Cell Reports, 9(2), 499-512.
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3

Fibrotic Fate Mapping of Immune and Stromal Cells

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Vav1-cre, FSP1-cre and ROSA26-enhanced yellow fluorescent protein (R26R-EYFP) mice were purchased from the Jackson Laboratory. Male Vav1-cre mice were crossed with female R26R-EYFP mice to generate Vav1-EYFP mice in which Vav1+ cells (mainly hematopoietic cells/macrophages) were labeled in EYFP to determine their fate such as trans-differentiation into fibroblasts. Similarly, FSP1-EYFP mice were generated by breeding FSP1-cre and R26R-EYFP mice to label FSP1+ fibroblasts. Genotyping was performed by polymerase chain reaction according to the protocols provided by the Jackson Laboratory. The transgenic mice of two months were used for experiments. Both male and female mice were used, and no sex difference in lineage tracing was detected. Teflon mandrels were implanted subcutaneously. After 1 week or 4 weeks, the Teflon mandrels were harvested for the histological analysis of the fibrotic conduits around the mandrels.
Qiu X., Lee B.L., Wong S.Y., Ding X., Xu K., Zhao W., Wang D., Sochol R., Dong N, & Li S. (2020). Cellular Remodeling of Fibrotic Conduit as Vascular Graft. Biomaterials, 268, 120565.
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4

Transgenic Mouse Models for Cancer Research

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Wild type C57BL/6 and FVB/n mice, and transgenic mice with ACTB-tdTomato-EGFP (Stock# 007676), FSP1-Cre (Stock# 012641), MMTV-PyMT (Stock# 002374), and MMTV-Neu (Stock# 002376) were obtained from The Jackson Laboratory (Bar Harbor, Maine). The Vimentin-Cre mouse was a kind gift from the laboratory of Dr. Schwabe at Columbia University. CB-17 SCID mice were obtained from Charles River (Wilmington, MA). All mouse strains obtained were bred in the animal facility at WCMC. All animal work was conducted in accordance with a protocol approved by the Institutional Animal Care and Use Committee at Weill Cornell Medical College.
The ACTB-tdTomato-EGFP and FSP1-Cre mice were bred together to obtain double transgenic mice and then bred with MMTV-PyMT or MMTV-Neu mice to obtain the Tri-PyMT and Tri-Neu triple transgenic mice, respectively. Double transgenic male mice carrying ACTB-tdTomato-EGFP and MMTV-PyMT alle were crossed with the Vimentin-Cre mice to obtain the Tri-PyMT-Vim triple transgenic mice. Genotyping for each transgenic line was performed following the standardized protocols as described in the website of The Jackson Laboratory. Genotyping for Vimentin-Cre was done using forward primer 5′-CCCCTTCCTCACTTCTTTCC and reverse primer 5′-ATGTTTAGCTGGCCCAAATG.
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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5

Conditional Twist1 Deletion in Fibroblasts

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Mice with conditional deletion of Twist1 were generated by crossing FSP1-Cre-expressing mice (FSP1-Cre from Jackson Laboratory, Bar Harbor, ME, stock no. 012641)55 (link) with Twist1fl/fl (Twist1 flox/flox mice from MMRRC, stock no. 16842). Twist1fl/fl mice were backcrossed to a more tenth-generation congenic Balb/c background. FSP1-Cre;Twist1fl/fl mice, which have a fibroblast-specific Twist1 Knock-out (Twist1 K/O). 10 μg of Recombinant TNC (Millipore Cat No. CC065) or PBS was injected in wound area at 3−5 days after 5-mm biopsy punch skin wound. The mice were sacrificed after remodeling phase onset of excisional skin wound.
Yeo S.Y., Lee K.W., Shin D., An S., Cho K.H, & Kim S.H. (2018). A positive feedback loop bi-stably activates fibroblasts. Nature Communications, 9, 3016.
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6

Generation and Validation of Conditional KLF4 Knockout Mice

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Rosa26CreER/KLF4(flox) mice and Fsp-1-Cre/KLF4 (flox) mice were generated by crossing Rosa26CreER (NCI, Stock#: 01XAB) and Fsp-1-Cre (Jackson, Stock#: 012641) with KLF4(flox) mice respectively. Rosa26CreER/KLF4(flox)/β-actin-EGFP mice were generated by crossing Rosa26CreER/KLF4(flox) mice with β-actin-EGFP mice (Jackson, Stock#: 006567). KLF4 knockout in RosaCre26ER/KLF4(flox) mice was induced by daily intraperitoneal injection of tamoxifen (TAM, Sigma, 50 mg/kg) for 5 consecutive days. Sunflower seed oil was used as a control. Mice were bred and used in specific pathogen-free facilities according to the Animal Care and Use Committee Guidelines of the University of South Carolina. In the wound healing, the control and mutant mice were littermates with mixed sex at a similar six to eight week age range.
Ou L., Shi Y., Dong W., Liu C., Schmidt T.J., Nagarkatti P., Nagarkatti M., Fan D, & Ai W. (2015). Kruppel like factor KLF4 facilitates cutaneous wound healing by promoting fibrocyte generation from myeloid-derived suppressor cells. The Journal of investigative dermatology, 135(5), 1425-1434.
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7

Generation and Validation of Conditional KLF4 Knockout Mice

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Rosa26CreER/KLF4(flox) mice and Fsp-1-Cre/KLF4 (flox) mice were generated by crossing Rosa26CreER (NCI, Stock#: 01XAB) and Fsp-1-Cre (Jackson, Stock#: 012641) with KLF4(flox) mice respectively. Rosa26CreER/KLF4(flox)/β-actin-EGFP mice were generated by crossing Rosa26CreER/KLF4(flox) mice with β-actin-EGFP mice (Jackson, Stock#: 006567). KLF4 knockout in RosaCre26ER/KLF4(flox) mice was induced by daily intraperitoneal injection of tamoxifen (TAM, Sigma, 50 mg/kg) for 5 consecutive days. Sunflower seed oil was used as a control. Mice were bred and used in specific pathogen-free facilities according to the Animal Care and Use Committee Guidelines of the University of South Carolina. In the wound healing, the control and mutant mice were littermates with mixed sex at a similar six to eight week age range.
Ou L., Shi Y., Dong W., Liu C., Schmidt T.J., Nagarkatti P., Nagarkatti M., Fan D, & Ai W. (2015). Kruppel like factor KLF4 facilitates cutaneous wound healing by promoting fibrocyte generation from myeloid-derived suppressor cells. The Journal of investigative dermatology, 135(5), 1425-1434.
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8

Genetically Engineered Murine Model

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All animal studies were approved by the Institutional Animal Care and Use Committee of Indiana University (IACUC # 20122). Male and female mice were fed with normal chow. Mice with skin lesion or loss of weight were monitored daily, with body weight measured weekly. FSP1-cre (Jackson Laboratory stock#000664), R26SmoM2 (shown as SmoM2 in the text, Jackson Laboratory stock#005130) and mTmG (Jackson Laboratory stock#007576) were purchased from The Jackson Laboratory, and breeding was set up according to the needs of the study (see details in results).
Gu D., Soepriatna A.H., Zhang W., Li J., Zhao J., Zhang X., Shu X., Wang Y., Landis B.J., Goergen C.J, & Xie J. (2023). Activation of the Hedgehog signaling pathway leads to fibrosis in aortic valves. Cell & Bioscience, 13, 43.
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9

Transgenic Mouse Models for Cancer Research

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Wild type C57BL/6 and FVB/n mice, and transgenic mice with ACTB-tdTomato-EGFP (Stock# 007676), FSP1-Cre (Stock# 012641), MMTV-PyMT (Stock# 002374), and MMTV-Neu (Stock# 002376) were obtained from The Jackson Laboratory (Bar Harbor, Maine). The Vimentin-Cre mouse was a kind gift from the laboratory of Dr. Schwabe at Columbia University. CB-17 SCID mice were obtained from Charles River (Wilmington, MA). All mouse strains obtained were bred in the animal facility at WCMC. All animal work was conducted in accordance with a protocol approved by the Institutional Animal Care and Use Committee at Weill Cornell Medical College.
The ACTB-tdTomato-EGFP and FSP1-Cre mice were bred together to obtain double transgenic mice and then bred with MMTV-PyMT or MMTV-Neu mice to obtain the Tri-PyMT and Tri-Neu triple transgenic mice, respectively. Double transgenic male mice carrying ACTB-tdTomato-EGFP and MMTV-PyMT alle were crossed with the Vimentin-Cre mice to obtain the Tri-PyMT-Vim triple transgenic mice. Genotyping for each transgenic line was performed following the standardized protocols as described in the website of The Jackson Laboratory. Genotyping for Vimentin-Cre was done using forward primer 5′-CCCCTTCCTCACTTCTTTCC and reverse primer 5′-ATGTTTAGCTGGCCCAAATG.
Fischer K.R., Durrans A., Lee S., Sheng J., Li F., Wong S., Choi H., El Rayes T., Ryu S., Troeger J., Schwabe R.F., Vahdat L.T., Altorki N.K., Mittal V, & Gao D. (2015). EMT is not required for lung metastasis but contributes to chemoresistance. Nature, 527(7579), 472-476.
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