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Nanozoomer scanner 2.0rs

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The Nanozoomer Scanner 2.0RS is a high-performance digital slide scanning system designed for rapid and reliable image acquisition of microscope slides. It features a high-resolution camera, automated slide handling, and advanced imaging algorithms to capture detailed digital representations of specimen samples.

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Lab products found in correlation

Ab5535, by Merck Group (2 mentions) Zo622, by Agilent Technologies (2 mentions) Tcs sp5, by Leica (2 mentions) Ab13970, by Abcam (2 mentions) Stellaris 5 microscope, by Leica (1 mentions)

Spelling variants of this product

NanoZoomer (461 citations) Nanozoomer scanner (61 citations) NanoZoomer 2.0 (45 citations) NanoZoomer-2.0RS digital scanner (3 citations)

5 protocols using nanozoomer scanner 2.0rs

1

Immunohistochemistry and Immunofluorescence Analysis of Tissue Samples

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Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections as described in 45 (link). Primary anti-Ki67 polyclonal antibody (clone SP6; M3062, Spring Bioscience) was from Spring Bioscience.
Immunofluorescences on PFA-fixed paraffin embedded tissue slices was performed as in 45 (link). Primary antibodies were anti-cytokeratin (wide spectrum screening, ZO622; Dako), anti-HNF4α (sc-6556; Santa Cruz Biotechnology), anti-SOX9 (AB5535; Millipore) and anti-GFP (ab13970; abcam). Samples were counterstained with ProLong-DAPI (Molecular Probes, Life Technologies) to label cell nuclei. Confocal images were obtained with a Leica TCS SP5 equipped with a CCD camera. Bright field images were obtained with a Nanozoomer Scanner 2.0RS (Hamamatsu).
Zanconato F., Battilana G., Forcato M., Filippi L., Azzolin L., Manfrin A., Quaranta E., Di Biagio D., Sigismondo G., Guzzardo V., Lejeune P., Haendler B., Krijgsveld J., Fassan M., Bicciato S., Cordenonsi M, & Piccolo S. (2018). Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4. Nature medicine, 24(10), 1599-1610.
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2

Quantifying Aortic Root Atherosclerosis in Mice

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Atherosclerosis progression was measured in euthanized mice at the end of the intervention, as previously described [31 (link)] (n = 5–6 per group). Briefly, the heart linked to the ascending aorta was carefully removed and snap-frozen at −80 °C. Peripheral fat was removed from defrosted heart samples, under a binocular magnifier, and samples were embedded in OCT and frozen at −80 °C for serial 10 μm-thick cryosectioning. Serial cross-sections of three valve leaflets from the aortic root were sectioned (five sections per slide, from 200 to 800 μm from the base of the heart to the aortic arch). The slides were stained with Oil Red O (ORO) to visualize neutral lipids and counterstained with hematoxylin. Images of all stained slides were captured with a NanoZoomer scanner 2.0 RS (Hamamatsu, Hamamatsu city, Japan). The areas of lesions were quantified using ImageJ software for image analysis and represented as the average of the lipid-stained areas for each distance from the heart [31 (link)].
Mérian J., Ghezali L., Trenteseaux C., Duparc T., Beuzelin D., Bouguetoch V., Combes G., Sioufi N., Martinez L.O, & Najib S. (2023). Intermittent Fasting Resolves Dyslipidemia and Atherogenesis in Apolipoprotein E-Deficient Mice in a Diet-Dependent Manner, Irrespective of Sex. Cells, 12(4), 533.
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3

Immunostaining Protocols for Cell and Tissue Analyses

Cited 1 time
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Immunofluorescence on PFA-fixed cells, on PFA-fixed paraffin-embedded tissue slices and on OCT(Sigma-Aldrich)-embedded tissue slices was performed as previously described57 (link). Samples were counterstained with ProLong-DAPI (Termo Fisher Scientific, P36971). Confocal images were acquired on a Leica STELLARIS 5 microscope, using LASX software (version 4.1.0.23081). Superresolution images were obtained with the LIGHTNING module. 3D reconstructions were obtained from Z-stacks using the LASX software. All images were analyzed using ImageJ software (version 2.0.0-rc-69/1.52i). Primary and secondary antibodies used for the immunofluorescence staining are listed in Supplementary Table 4. YAP/TAZ, p-MLC2, p-FAK, cGAS and F-Actin immunofluorescence stainings were performed on OCT-embedded tissue slices. Elastic lamellae autofluorescence was detected upon excitation with the 488 laser line.
Immunohistochemical staining was performed on PFA-fixed, paraffin-embedded tissue sections as previously described57 (link). Brightfield images were obtained with a Nanozoomer Scanner 2.0RS (Hamamatsu), equipped with the NDPscan3.1 software. Primary antibodies used in immunostaining are listed in Supplementary Table 4.
Quantifications from immunofluorescence and immunohistochemistry staining were performed using ImageJ software (version 2.0.0-rc-69/1.52i).
Sladitschek-Martens H.L., Guarnieri A., Brumana G., Zanconato F., Battilana G., Xiccato R.L., Panciera T., Forcato M., Bicciato S., Guzzardo V., Fassan M., Ulliana L., Gandin A., Tripodo C., Foiani M., Brusatin G., Cordenonsi M, & Piccolo S. (2022). YAP/TAZ activity in stromal cells prevents ageing by controlling cGAS-STING. Nature, 607(7920), 790-798.
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4

Histological Analysis of Adipose Tissues

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Tissue histology was performed as previously described [28 (link)]. Briefly, liver, subcutaneous white adipose tissue (SC-WAT), and brown adipose tissue (BAT) were fixed in 4% paraformaldehyde for 24 h at room temperature, dehydrated, and embedded into paraffin. The tissues were sectioned into 5 μm slices. The sections were incubated for 30 min in Mayer’s hematoxylin solution, rinsed with distilled water for 5 min, and then incubated in saturated lithium carbonate solution for 15 s. The sections were then rinsed again with distilled water for 3 min and finally placed in 0.5% alcoholic eosin solution for 30 s. The slides were scanned with a NanoZoomer scanner 2.0 RS (Hamamatsu, Hamamatsu city, Japan) at 40× magnification. The images shown are representative results of at least three biological replicates.
Mérian J., Ghezali L., Trenteseaux C., Duparc T., Beuzelin D., Bouguetoch V., Combes G., Sioufi N., Martinez L.O, & Najib S. (2023). Intermittent Fasting Resolves Dyslipidemia and Atherogenesis in Apolipoprotein E-Deficient Mice in a Diet-Dependent Manner, Irrespective of Sex. Cells, 12(4), 533.
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5

Immunohistochemistry and Immunofluorescence Analysis of Tissue Samples

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Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissue sections as described in 45 (link). Primary anti-Ki67 polyclonal antibody (clone SP6; M3062, Spring Bioscience) was from Spring Bioscience.
Immunofluorescences on PFA-fixed paraffin embedded tissue slices was performed as in 45 (link). Primary antibodies were anti-cytokeratin (wide spectrum screening, ZO622; Dako), anti-HNF4α (sc-6556; Santa Cruz Biotechnology), anti-SOX9 (AB5535; Millipore) and anti-GFP (ab13970; abcam). Samples were counterstained with ProLong-DAPI (Molecular Probes, Life Technologies) to label cell nuclei. Confocal images were obtained with a Leica TCS SP5 equipped with a CCD camera. Bright field images were obtained with a Nanozoomer Scanner 2.0RS (Hamamatsu).
Zanconato F., Battilana G., Forcato M., Filippi L., Azzolin L., Manfrin A., Quaranta E., Di Biagio D., Sigismondo G., Guzzardo V., Lejeune P., Haendler B., Krijgsveld J., Fassan M., Bicciato S., Cordenonsi M, & Piccolo S. (2018). Transcriptional addiction in cancer cells is mediated by YAP/TAZ through BRD4. Nature medicine, 24(10), 1599-1610.
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