N oleoylethanolamine

Fura-2 AM was purchased from Invitrogen (Carlsbad, CA). 5-Iodoresiniferatoxin (I-RTX) was purchased from Tocris Cookson Inc. (Ellisville, MO). Ruthenium red was purchased from Sigma-Aldrech (St. Louis, MO). 2-Aminoethoxydiphenyl borate (2-APB) and 4-alpha-Phorbal 12,13-didecanoate (4-alpha PDD) were purchased from Enzo Life Sciences (Farmingdale, NY). Each of the following N-acyl amides were purchased from Cayman Chemical (Ann Arbor, MI): N-arachidonoyl ethanolamine (Anandamide; AEA), N-oleoyl ethanolamine (OEA), N-palmitoyl ethanolamine (PEA), N-linoleoyl ethanolamine (LEA), N-docosahexaenoyl ethanolamine (DEA), N-arachidonyl glycine (NAGly), and 2-AG All other reagent-grade N-acyl amides were synthesized in house as previously described (Tan et al., 2010 (link)). Unless otherwise indicated, all compounds were stored in 100% ethanol, however, assay solutions were made fresh in DMSO prior to every assay. Chemicals used for mass spectrometry were HPLC-grade and include methanol, water, ammonium acetate, and acetic acid. HPLC water and methanol were purchased from VWR International (Plainview, NY). Ammonium acetate and acetic acid were purchased from Sigma Aldrish (St. Louis, MO).