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Wheat starch

Manufactured by Merck Group
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Sourced in United States, United Kingdom, Spain

Wheat starch is a fine, white, powdery substance extracted from wheat grains. It serves as a core ingredient in various laboratory applications, providing a reliable and consistent source of carbohydrates for scientific research and analysis.

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Lab products found in correlation

Potato starch, by Merck Group (4 mentions) Corn starch, by Merck Group (4 mentions) Pichia pastoris strain x 33, by Thermo Fisher Scientific (1 mentions) Pgapzαa vector, by Thermo Fisher Scientific (1 mentions) Amicon ultra centrifugal filter, by Merck Group (1 mentions) Rneasy midi kit, by Qiagen (1 mentions) Qiaprep spin miniprep kit, by Qiagen (1 mentions) Wheat gluten, by Merck Group (1 mentions) Sulfuric acid, by Scharlab (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Milli q water system, by Merck Group (1 mentions) Waxy corn starch, by Merck Group (1 mentions) Tae buffer, by Merck Group (1 mentions) Soluble starch, by Merck Group (1 mentions) Rice starch, by Merck Group (1 mentions) Plasticizer glycerol, by Thermo Fisher Scientific (1 mentions) Pectinex ultra pulp, by Novozymes (1 mentions) Pectinex ultra sp l, by Novozymes (1 mentions) Srebp1, by Abcam (1 mentions) Acetyl coa carboxylase, by Abcam (1 mentions)

14 protocols using wheat starch

1

Protein Expression in Pichia pastoris

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Escherichia coli DH5α strain (Sangon Biotech, Shanghai) was used for gene cloning and plasmid maintenance throughout the study, and Pichia pastoris X33 strain (Invitrogen, Carlsbad, CA, USA) was used for protein expression. The pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) was used for gene cloning. Potato starch, wheat starch, corn starch, amylose (potato) and amylopectin (potato) were obtained from Sigma-Aldrich (St. Louis, MO). The Mix (Green) for PCR amplification was from TsingKe (Beijing, China). The QIAprep Spin Miniprep Kit and RNeasy@ Midi Kit were obtained from Qiagen (Valencia, CA). SMART® cDNA Library Construction Kit, Advantage® 2 PCR Kit and the Talon metal affinity resin were purchased from Clontech Laboratories, Inc. (Mountain View, CA). The Amicon@ ultra centrifugal filters were obtained from Millipore (Billerica, MA). All other reagents were purchased from general commercial suppliers and used without further purification.
Yi Z., Fang Y., He K., Liu D., Luo H., Zhao D., He H., Jin Y, & Zhao H. (2018). Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter. Microbial Cell Factories, 17, 30.
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2

Development of Serish Root Powder Formulations

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The Serish root powders were attained from the local medical plant market, Mashhad, Iran. Wheat gluten (catalog number G-5004, ≥80% protein) and wheat starch (catalog number G-5004, ≥98% starch) were procured from Sigma–Aldrich (Gillingham, Dorset, UK). Propylene glycol was purchased from J.T. Baker Chemical Co., Phillipsburg, NJ, USA. Glycerol (Superol glycerin USP) was purchased from Proctor and Gamble Co., Cincinnati, OH, USA. Sorbitol (Neosorb, 70 g/100 g solids content) was purchased from Roquette Corporation, Gurnee, IL, USA. All other chemicals, reagents and solvents were of analytical grade.
Pourfarzad A., Ahmadian Z, & Habibi-Najafi M.B. (2018). Interactions between polyols and wheat biopolymers in a bread model system fortified with inulin: A Fourier transform infrared study. Heliyon, 4(12), e01017.
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3

Characterization of Sugarcane Bagasse Black Liquor

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Soda black liquor of sugarcane bagasse was received from the Pars paper mill (Khuzestan, Iran). Total solids, total lignin, Klason lignin, mineral element and organic extractive were 47.7, 38.3, 28.0, 24.0, and 20.2 wt%, respectively. Wheat starch was purchased from Sigma Aldrich (Stockholm, Sweden) and was used in experiments as supplied. Sulfuric acid (97%) supplied by Scharlau (Madrid, Spain), sodium hydroxide (98%) supplied by Sigma Aldrich (Stockholm, Sweden), and glycerol supplied by VWR Chemicals BDH (Leverantor, Belgium) were also used in the experiments.
Mousavi S.N., Nazarnezhad N., Asadpour G., Ramamoorthy S.K, & Zamani A. (2021). Ultrafine Friction Grinding of Lignin for Development of Starch Biocomposite Films. Polymers, 13(12), 2024.
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4

Synthesis and Characterization of Cyclodextrin Derivatives

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α-Cyclodextrin (α-CD) and γ-Cyclodextrin (γ-CD) were purchased from Wacker Chemie AG, Munich, Germany. Dodecyl-β-maltoside (β-DDM) and Dodecyl-β-glucoside were sourced from Anatrace Inc., Maumee, OH, and wheat starch from Sigma-Aldrich, St. Louis, MO. Synthesis of dodecyl-β-maltooctaoside (C12G8) is described by Rather et al. (2015 ). All other chemical and solvents were HPLC grade and purchased from VWR International, Stockholm, Sweden.
Ara K.Z., Linares-Pastén J.A., Jönsson J., Viloria-Cols M., Ulvenlund S., Adlercreutz P, & Karlsson E.N. (2020). Engineering CGTase to improve synthesis of alkyl glycosides. Glycobiology, 31(5), 603-612.
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5

Characterization of Modified Starches

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Potato starch (20 wt% amylose); wheat starch (28 wt% amylose); corn starch wild type (approx. 27% amylose); waxy corn starch (76 wt% amylose) and low amylose corn starch (0–1 wt% amylose) and TAE buffer were purchased from Sigma-Aldrich Chemie GmbH (Munich, Germany). Modified Starches: Alimentamyl 2002 (distarch phosphate); Amylex 20/20 (depolymerized Potato starch); Fibraffin K105 (cationic starch ether); Licocat P (quaternary cationic starch solution); Sobocat (quaternary starch ether); Sobotex 5305 NN (starch acetate) and Sobotex CM (starch ether) were provided by Südstärke GmbH (Schrobenhausen, Germany). The undefined starch was purchased in a supermarket in Germany. Double deionized water from a Milli-Q water system (Merck Millipore, Billerica, MA, USA) was used throughout all experiments.
Meiling T.T., Cywiński P.J, & Bald I. (2016). White carbon: Fluorescent carbon nanoparticles with tunable quantum yield in a reproducible green synthesis. Scientific Reports, 6, 28557.
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6

Purified Maltooligosaccharides and Starch Substrates

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Maltooligosaccharides (purity ≥ 95%), including maltose (G2), maltotriose (G3), maltotetraose (G4), maltopentaose (G5), and maltohexaose (G6), were purchased from Wako Pure Chemical (Osaka, Japan).
The substrates (soluble starch, wheat starch, corn starch, potato starch and cassava starch) were acquired from the Sigma Chemical Co. (St. Louis, MO, USA).
Wang Y., Xie T., Yan G., Xue H., Zhao Z, & Ye X. (2024). Heterologous Expression and Characterization of a Novel Mesophilic Maltogenic α-Amylase AmyFlA from Flavobacterium sp. NAU1659. Applied biochemistry and biotechnology.
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7

Wheat Starch Hydrolysis Assay

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Hydrolysis activity was determined by the 3,5-dinitrosalicylic acid (DNS) reagent according to the method described in Ara et al. (2015) (link), using 1% (w/v) wheat starch (Sigma). 1 unit of hydrolytic activity is defined as 1 μmol of maltose formed per minute and all the reactions were run in triplicates.
Ara K.Z., Linares-Pastén J.A., Jönsson J., Viloria-Cols M., Ulvenlund S., Adlercreutz P, & Karlsson E.N. (2020). Engineering CGTase to improve synthesis of alkyl glycosides. Glycobiology, 31(5), 603-612.
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8

Starch Characterization and Comparison

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Corn starch (24.8% amylose, 0.020% phosphorus content, 11% moisture content), wheat starch (24.5% amylose, 0.038% phosphorus content, 9.6% moisture content) and rice starch (16.9% amylose, 0.030% phosphorus content, 9.2% moisture content) were purchased from Sigma-Aldrich (Madrid, Spain). Potato starch (20.5% amylose, 0.043% phosphorus content, 15.1% moisture content) was provided by Across Organics (Geel, Belgium), and the plasticizer glycerol was supplied by Fisher Chemical (Geel, Belgium). All chemicals were used without further purification.
Domene-López D., García-Quesada J.C., Martin-Gullon I, & Montalbán M.G. (2019). Influence of Starch Composition and Molecular Weight on Physicochemical Properties of Biodegradable Films. Polymers, 11(7), 1084.
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9

Enzymatic Extraction of Citrus Unshiu

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Citrus unshiu were purchased from a local market at Jeju in the Republic of Korea. The pectinases, Pectinex Ultra SP-L and Pectinex Ultra Pulp, were purchased from Novozymes A/S (Bagsvaerd, Denmark). Wheat starch was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Park H.Y., Ryu A.R., Kim H.R., Shin K.S., Hong J.S, & Choi H.D. (2021). Effects of Citrus Peel Hydrolysates on Retrogradation of Wheat Starch. Foods, 10(10), 2422.
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10

Molecular Mechanisms of Lipid Regulation

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Wheat starch, 4 kDa fluorescein isothiocyanate (FITC)-dextran, and NaCl were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies to western blot, such as peroxisome proliferator-activated receptor-γ (PPAR-γ), peroxisome proliferator-activated receptor-α (PPAR-α), sterol regulatory element-binding protein 1 (SREBP-1), acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), monocyte chemoattractant protein-1 (MCP-1), interleukin-1β (IL-1β), interleukin-6 (IL-6), TLR-4, tumor necrosis factor-α (TNF-α), and β-actin, were purchased from Abcam (Cambridge, MA, USA). Mouse and rabbit secondary antibodies were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Tissue-Tek® O.C.T.™ Compound was purchased from Sakura (Alphen aan den Rijn, Netherlands)
Do M.H., Lee H.B., Lee E, & Park H.Y. (2020). The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder. Nutrients, 12(2), 301.
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