Anti ezh2 clone 11 ezh2

We selected 54 (Additional file 1: Table S1) malignant and 40 benign (Additional file 2: Table S2) salivary gland tumors from the archives of the First Department of Pathology and Experimental Cancer Research, Semmelweis University (Budapest, Hungary) and Pathology Department of National Institute of Oncology (Budapest, Hungary). The study was approved by the ethics committee of the Semmelweis University. The tumors were diagnosed according to standard diagnostic criteria and immunohistochemical staining. Formalin-fixed paraffin-embedded tissue was used for the immunohistochemical reactions. Staining was performed using an automated Leica Bond immunostainer, with the Leica Bond Polymer refine detection system and 3,3′ Diaminobenzidine (DAB) as the chromogen. Antigen retrieval was achieved with Bond Epitope Retrieval Solution 2 (high pH) for 20 min. The primary antibody was a mouse monoclonal anti-EZH2 (clone 11/EZH2) from BD Biosciences (San Jose CA, USA) (dilution 1:100). The reaction resulted in nuclear staining. Scores were assigned based on the density of positivity by using negative (score =0, < 5 % of nuclei staining); weak (score = 1, 5–10 % of nuclei staining); moderate (score = 2, 11–50 % of nuclei staining); and strong (score = 3; >50 % of nuclei staining).