Brain sections were immunostained with anti-laminin γ1 (Abcam, AB3297, 1:200), anti-laminin-111 (Sigma, L9393, 1:1000), anti-laminin α2 (Sigma, L0663, 1:200), anti-laminin α4 (R&D system, AF3837, 1:200), anti-AQP4 (Millipore, AB3594, 1:500), anti-occludin (Invitrogen, 71-1500, 1:500), anti-ZO-1 (Invitrogen, 40-2200, 1:500), anti-Claudin-5 (Invitrogen, 34-1600, 1:500), anti-CD31 (BD Pharmingen, 550274, 1:200), anti-PDGFRβ (eBioscience, 14-1402-82, 1:200 and Cell Signaling, 3169S, 1:200), anti-CD13 (BD Pharmingen, 558744, 1:200), anti-Cre recombinase (Novagen, 69050, 1:2000), or anti-Ki67 (Millipore, AB9260, 1:1000) antibodies overnight at 4°C, followed by fluorescent secondary antibodies (Invitrogen) for 1 hour at room temperature. FITC-conjugated SMA (Sigma, F3777, 1:1000) was used to study pericyte differentiation and distinguish capillaries and large vessels, given that SMA is expressed in arterioles and arteries but not capillaries under normal conditions. After mounting, the brain sections were examined and photographed with an Axiovert 200 (Zeiss) microscopy or Leica confocal microscopy. For quantification, at least 9 random images (from at least 3 animals) with GFP or Cre signal at 200X magnification were taken and the mean fluorescence intensity was analyzed.
Ab3297
Manufactured by Merck Group
The AB3297 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory applications. The core function of the AB3297 is to facilitate standard laboratory processes, but a detailed description of its intended use is not available.
Automatically generated - may contain errors
Lab products found in correlation
F3777, by Merck Group (2 mentions)
L0663, by R&D Systems (2 mentions)
Af3837, by Merck Group (2 mentions)
Ab3594, by Thermo Fisher Scientific (2 mentions)
Fitc conjugated sma, by Merck Group (2 mentions)
Fluorescent secondary antibody, by Thermo Fisher Scientific (2 mentions)
Ab9260, by Merck Group (2 mentions)
Anti pdgfrβ, by Thermo Fisher Scientific (2 mentions)
Anti occludin, by Thermo Fisher Scientific (2 mentions)
Anti claudin 5, by Thermo Fisher Scientific (2 mentions)
L9393, by Merck Group (2 mentions)
Anti zo 1, by Thermo Fisher Scientific (2 mentions)
Axiovert 200, by Zeiss (2 mentions)
2 protocols using ab3297
1
Immunohistochemical Analysis of Brain Vasculature
2
Immunohistochemical Analysis of Brain Vasculature
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Brain sections were immunostained with anti-laminin γ1 (Abcam, AB3297, 1:200), anti-laminin-111 (Sigma, L9393, 1:1000), anti-laminin α2 (Sigma, L0663, 1:200), anti-laminin α4 (R&D system, AF3837, 1:200), anti-AQP4 (Millipore, AB3594, 1:500), anti-occludin (Invitrogen, 71-1500, 1:500), anti-ZO-1 (Invitrogen, 40-2200, 1:500), anti-Claudin-5 (Invitrogen, 34-1600, 1:500), anti-CD31 (BD Pharmingen, 550274, 1:200), anti-PDGFRβ (eBioscience, 14-1402-82, 1:200 and Cell Signaling, 3169S, 1:200), anti-CD13 (BD Pharmingen, 558744, 1:200), anti-Cre recombinase (Novagen, 69050, 1:2000), or anti-Ki67 (Millipore, AB9260, 1:1000) antibodies overnight at 4°C, followed by fluorescent secondary antibodies (Invitrogen) for 1 hour at room temperature. FITC-conjugated SMA (Sigma, F3777, 1:1000) was used to study pericyte differentiation and distinguish capillaries and large vessels, given that SMA is expressed in arterioles and arteries but not capillaries under normal conditions. After mounting, the brain sections were examined and photographed with an Axiovert 200 (Zeiss) microscopy or Leica confocal microscopy. For quantification, at least 9 random images (from at least 3 animals) with GFP or Cre signal at 200X magnification were taken and the mean fluorescence intensity was analyzed.
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