Epithelial cells cultured on Transwell membrane filters were fixed with cold methanol and subjected to immunofluorescence labeling as previously described (101 (link), 117 (link)). Immunolabeled cell monolayers were examined by confocal microscopy using a Leica TCS-SP spectral laser-scanning confocal microscope. Epithelial cells infected with RFP-labeled RSV were visualized using a Lumen200 epifluorescence microscope (Prior, Cambridge, UK), and the number of RFP-positive cells was quantified. Additionally, fluorescent-focused unit (FFU) assay was performed to quantitatively analyze of RFP-positive cells by ImageJ (112 (link)).
Tcs sp spectral laser scanning confocal microscope
Manufactured by Leica
The TCS-SP spectral laser-scanning confocal microscope is a scientific instrument designed for high-resolution imaging of microscopic samples. It utilizes a laser scanning system and a spectral detection method to capture detailed images of specimens. The core function of this microscope is to provide researchers with a versatile tool for advanced microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tcs sp spectral laser scanning confocal microscope
1
Immunofluorescence Imaging of Epithelial Cells
2
Immunofluorescent Staining and Confocal Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunofluorescent staining, cells were fixed in 4% paraformaldehyde and subjected to immunofluorescence labeling as previously described (33 (link)–35 (link)). Immunolabeled cell monolayers were examined using a Leica TCS-SP spectral laser scanning confocal microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!