Cell migration was performed using the Radius™ 24-well from Cell Biolabs (San Diego, CA). Briefly, cells were seeded on Radius cell migration plates and allowed to form monolayers. Circular gaps were generated by removing the gels and cells were treated with DMSO or LM11 for 24 hours. To compare differences in the migratory gap, phase-contrast images were captured at the same size using a Zeiss LSM-510 inverted microscope (Zeiss, Germany) and gap closure was analyzed using ImageJ. Cell migration velocity was calculated and statistically analyzed from three independent experiments. Cell invasion was performed using a Matrigel-coated modified Boyden chamber (BD biosciences, San Jose, CA) as described previously [45 (link)–47 (link)]. After incubation for 24 hours, cells on the upside were removed using cotton swabs, and the invading cells on the lower side were fixed and stained with 0.2% crystal violet. Numbers of the invading cells in six randomly selected fields were counted in each experiment using a Zeiss LSM-510 inverted microscope.
Matrigel coated modified boyden chamber
Manufactured by BD
Sourced in United States
The Matrigel-coated modified Boyden chamber is a laboratory equipment used for cell migration and invasion assays. It consists of an upper and lower chamber separated by a porous membrane, with the upper chamber coated with Matrigel, a gelatinous protein mixture. This setup allows for the assessment of a cell's ability to migrate through the Matrigel barrier and into the lower chamber.
Automatically generated - may contain errors
3 protocols using matrigel coated modified boyden chamber
1
Cell Migration and Invasion Assays
2
Cell Motility Assays and Rac Activity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ror2 internalization at endogenous levels using cell surface biotinylation was examined as described previously22 (link). Rac activity was assayed using GST-CRIB as described16 (link)58 (link). Quantification data was calculated based on at least three blots from different experiments. Migration capabilities of KKLS, HeLaS3, and A549 cells were measured using a modified Boyden chamber10 (link)14 (link). When necessary, 25 μg/ml mAb5A16, pAb5a-5, or anti-GST antibody were used. The invasive potential of the cells was analyzed using a matrigel-coated modified Boyden chamber (BD Biosciences) as described previously10 (link)11 (link).
3
Cell Migration and Invasion Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Migration and invasion assays were performed using a modified Boyden chamber (8 µm pores;
Corning) and a Matrigel-coated modified Boyden chamber (8 µm pores; BD Biosciences, Franklin Lakes, NJ, USA), respectively as described previously (Kurayoshi et al, 2006; (link)Matsumoto et al, 2014) (link).
In the standard conditions, S2-CP8 cells (2.5 x 10 4 cells) were seeded in the upper side of Boyden Chamber. In GFP-expressing S2-CP8 cells, after 4 h (migration assay) or 24 h (invasion assay, except for Figure 6E) incubation with control ASO, 122 cells (average) and 126 cells (average), respectively, were observed in the lower side chamber in the one field of view under fluorescence microscope (BZ-9000, Keyence, Osaka, Japan) using a 10x air objective. In Figure 6E, cells were observed after 20 h incubation with ASO. Migration and invasion rates of cells expressing ARL4C, IQGAP1, and MMP14 mutants were calculated as the percentages of the same cells transfected with control ASO or siRNA.
Corning) and a Matrigel-coated modified Boyden chamber (8 µm pores; BD Biosciences, Franklin Lakes, NJ, USA), respectively as described previously (Kurayoshi et al, 2006; (link)Matsumoto et al, 2014) (link).
In the standard conditions, S2-CP8 cells (2.5 x 10 4 cells) were seeded in the upper side of Boyden Chamber. In GFP-expressing S2-CP8 cells, after 4 h (migration assay) or 24 h (invasion assay, except for Figure 6E) incubation with control ASO, 122 cells (average) and 126 cells (average), respectively, were observed in the lower side chamber in the one field of view under fluorescence microscope (BZ-9000, Keyence, Osaka, Japan) using a 10x air objective. In Figure 6E, cells were observed after 20 h incubation with ASO. Migration and invasion rates of cells expressing ARL4C, IQGAP1, and MMP14 mutants were calculated as the percentages of the same cells transfected with control ASO or siRNA.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!