Dna amplification reagents

DNA amplification reagents and DNA purification kits were from Qiagen (Valencia, CA). Bacterial media were from Becton Dickinson (Franklin Lakes, NJ). Electrophoresis reagents were from Bio-Rad (Hercules, CA). Endonucleases and other enzymes were from New England Biolabs (Ipswich, MA). Other biochemicals were from Sigma-Aldrich (St. Louis, MO), otherwise mentioned in the text. All reagents used were analytical or molecular biology grade.

DNA amplification reagents and purification kits were acquired from Qiagen (Germantown, MD, USA). Bacterial culture medium components were obtained from Becton, Dickinson and Company (Franklin Lakes, NJ, USA). Protein analysis and purification reagents were supplied by Bio-Rad Laboratories (Hercules, CA, USA), GE Healthcare Bio-Sciences (Pittsburgh, PA, USA), and Qiagen. Endonucleases and other enzymes were available from New England Biolabs (Ipswich, MA, USA). Unless otherwise mentioned, additional reagents were provided by Sigma-Aldrich (St. Louis, MO, USA). All materials were biochemical or biotechnological research grade.