Igg1 pc7

hBM-MSCs and hWJ-MSCs were detached and counted; 1 × 10⁵ cells were incubated at RT for 20 min with the following directly conjugated mouse-anti human antibodies: CD90 FITC (Beckman Coulter, Fullerton, CA, USA), CD73 APC (Miltenyi Biotec, Gladbach, Germany), CD105 PE (Beckman Coulter, Fullerton, CA, USA), CD45 PC7 (Beckman Coulter, Fullerton, CA, USA), HLA class-II FITC (Beckman Coulter, Fullerton, CA, USA), CD14 PC7 (Beckman Coulter, Fullerton, CA, USA), and CD34 PE (Beckman Coulter, Fullerton, CA, USA). The isotype-matched immunoglobulins IgG1 FITC (Beckman Coulter, Fullerton, CA, USA), IgG1 PE (Beckman Coulter, Fullerton, CA, USA), IgG1 APC (Beckman Coulter, Fullerton, CA, USA), and IgG1 PC7 (Beckman Coulter, Fullerton, CA, USA) were used as negative controls under the same conditions. At least 15,000 total events were acquired with a BD FACSVerse flow cytometer (Becton Dickinson, BD, NJ, USA). Further analysis and plots were generated using the BD FACSuite analysis software. Statistics are summarized in Table S1 (Supplementary Materials).

Fluorescence-activated cell sorting was used to measure cell counts for leukocytes, neutrophils, T cells, CD4+T cells, CD8+T cells, NK cells, and B cells (anti-CD45 FITC; anti-CD56 PE, anti-CD16 PE, anti-CD19 ECD, anti-CD3 PC5, IgG1 PC7, anti-CD4 PE, anti-CD8 PCD, anti-CD3 PC5 [Beckman Coulter]).

Differential blood cell counts (XN9000, Sysmex, Norderstedt, Germany), PCT and CRP (Adivia Centaur XPT and Dimension Vista, Siemens Healthcare Diagnostics, Eschborn, Germany) were determined. Fluorescence-activated cell sorting was used to measure cell counts for leukocytes, T-cells, CD4+T-cells, CD8+T cells, NK cells and B cells as well as HLA-DR on T-cells (anti-CD45 FITC; anti-CD56 PE, anti-CD19 ECD, anti-CD3 PC5, IgG1 PC7, anti-CD4 PE, anti-CD8 PCD, anti-CD3 PC5, anti-HLA-DR-PE [FC500; Beckman Coulter]).