Fresh bovine blood was collected from a local abattoir in a sealed container and treated with 10,000 IUs of heparin sodium from porcine mucosa (Sigma Aldrich, Dorset, UK) in 50ml of 0.9% NaCl. To measure the PCV, blood samples were collected in capillary tubes by capillary action and one end was sealed with an inert wax. Tubes were spun in a Hawksley 127 haematocrit centrifuge (Hawksley and Sons Ltd., Brighton, UK) for 2 minutes and the PCV was read using a Hawksley micro-haematocrit reader. To haemodilute the blood samples to the appropriate PCV concentrations, varying volumes of 0.9% sodium chloride solution was added to the blood and the PCV was measured. Depending on the measured PCV, additional blood or saline was added to acquire the desired PCV for sensor assessment. Fifty millilitres of haemodiluted blood was collected and 1 ml blood samples were placed in absorption cell cuvettes with a 5 mm light path for spectral analysis (Hellma Analytics, Mullheim, Germany).
Heparin sodium from porcine mucosa
Manufactured by Merck Group
Sourced in United Kingdom
Heparin sodium from porcine mucosa is a product used in laboratory settings. It is a naturally occurring anticoagulant substance derived from porcine (pig) intestinal mucosa. This product is used in various in vitro and ex vivo applications that require an anticoagulant.
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Lab products found in correlation
2 protocols using heparin sodium from porcine mucosa
1
Bovine Blood Preparation for Sensor Evaluation
2
Characterizing Pump Flow Rates with Bovine Blood
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The high flow pumping using the larger upper pump and low flow pumping capability of the smaller lower pump were tested using the motorised docking station (in order to control RPM input). The pump was tested using fresh bovine blood collected from a local abattoir (Sandyford Abattoir, Paisley, UK) in a sealed container and treated with 10 000 IUs of heparin sodium from porcine mucosa (Sigma Aldrich, Dorset, UK) in 50 mL of 0.9% sodium chloride solution. Flow rates were set between 30 RPM and 75 RPM in accordance with realistic operational capabilities, and this was achieved by altering the voltage output of the power supply. For the upper and lower pumps, the inlet tubing was placed in a beaker containing 5 L and 2 L of blood, respectively, and the outlet tubing was placed in an empty beaker to measure volume of transferred blood. The flow rate was measured by calculating the time taken to transfer the blood through the pump—litres per minute was then subsequently calculated. Flow rate values were recorded at RPM values between 30 and 75 (Figure 5 ).
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